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LncRNA LEF1-AS1 Promotes Ovarian Cancer Development Through Interacting with miR-1285-3p
| Content Provider | Scilit |
|---|---|
| Author | Zhang, Yanan Ruan, Fang |
| Copyright Year | 2020 |
| Description | Background: Growing evidence indicates that long noncoding RNA (lncRNA) is a group of important regulator in cancer development. However, the correlation between lncRNA and ovarian cancer remains elusive. Here, we aimed to investigate the roles of LEF1-AS1 in ovarian cancer progression. Methods: LEF1-AS1 expression was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Survival rate was analyzed by Kaplan-Meier method. Cell Counting Kit-8 (CCK8) and colony formation assays were used for proliferation analysis. Transwell assay was utilized for analyses of migration and invasion. Luciferase reporter assay was performed to test the interaction between LEF1-AS1 and miR-1285-3p. Results: We showed that LEF1-AS1 expression was upregulated in ovarian cancer tissues compared with normal tissues. Besides, LEF1-AS1 level was positively correlated with lymph node metastasis and advanced stage. Enhanced expression of LEF1-AS1 may predict a poor prognosis. Moreover, LEF1-AS1 knockdown suppressed ovarian cancer cell proliferation, migration and invasion. Mechanistically, LEF1-AS1 exerted its oncogenic functions through interacting with miR-1285-3p to inhibit miRNA activity. Rescue assay validated that miR-1285-3p inhibitors abrogated LEF1-AS1-silencer-caused suppression of ovarian cancer progression. Conclusion: Our study revealed that LEF1-AS1 acts as a vital regulation in ovarian cancer progression. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997035/pdf https://www.dovepress.com/getfile.php?fileID=55741 |
| Ending Page | 694 |
| Page Count | 8 |
| Starting Page | 687 |
| ISSN | 11791322 |
| DOI | 10.2147/cmar.s227652 |
| Journal | Cancer Management and Research |
| Volume Number | 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Management and Research Oncology Lef1-as1 Mir-1285-3p Ovarian Cancer Progression |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oncology |
