NDLI: LncRNA TTN‑AS1 promotes endometrial cancer by sponging miR‑376a‑3p.

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LncRNA TTN‑AS1 promotes endometrial cancer by sponging miR‑376a‑3p.

Content Provider	Europe PMC
Author	Shen, Longde Wu, Yinyin Li, Ailu Li, Lichun Shen, Longyuan Jiang, Qiuxia Li, Qiuxia Wu, Zhifen Yu, Liji Zhang, Xiaohong
Copyright Year	2020
Abstract	Increasing research has demonstrated that lncRNAs participate in the development of multiple cancer types. However, the role of TTN-AS1 in endometrial cancer (EC) remains unknown. The present study aimed to explore the function of titin-antisense RNA1 (TTN-AS1) in EC progression and the underlying mechanisms. qRT-PCR was performed to assess the TTN-AS1 expression patterns in EC tissues and cell lines. Loss of function experiments were carried out to estimate the effects of TTN-AS1 on EC cell proliferation, migration and invasion. To reveal the underlying mechanisms, informatics tools were used to predict the targets. Rescue experiments were performed to investigate the TTN-AS1-regulated miR-376a-3p/pumilio homolog 2 (PUM2) axis involved. The results of the present study revealed that TTN-AS1 was highly expressed in both EC tissues and cell lines, and TTN-AS1 knockdown inhibited EC cell proliferation, migration and invasion. With respect to the mechanisms, miR-376a-3p was revealed to be targeted by TTN-AS1, and reversed the effects on EC development induced by TTN-AS1. In addition, PUM2 was positively regulated by TTN-AS1, and miR-376a-3p mediated the regulation between them. Furtherly, in vivo experiments confirmed the results. Collectively, TTN-AS1 enhanced EC cell proliferation and metastasis by targeting the miR-376a-3p/PUM2 axis, which may shed light on EC diagnosis and treatment.
ISSN	1021335X
Journal	Oncology Reports
Volume Number	44
PubMed Central reference number	PMC7448418
Issue Number	4
PubMed reference number	32945477
e-ISSN	17912431
DOI	10.3892/or.2020.7691
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-07-15
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Shen et al.
Subject Keyword	lncRNA TTN-AS1 endometrial cancer miR-376a-3p PUM2 cell proliferation
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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