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Preparation of an affinity cryogel column for lysozyme purification
| Content Provider | Scilit |
|---|---|
| Author | Veríssimo, Lizzy Ayra Alcântara Paganoto, Fernanda Spagnol Mol, Paula Chequer Gouveia Fontan, Rafael Da Costa Ilhéu Minim, Valéria Paula Rodrigues Minim, Luis Antonio |
| Copyright Year | 2017 |
| Description | Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design $2^{2}$ was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13%. Results showed that the affinity cryogel was a potential separation medium for LYZ purification. |
| Related Links | https://repositorio.unesp.br/bitstream/11449/169760/1/2-s2.0-85019686552.pdf |
| Ending Page | 1982 |
| Page Count | 10 |
| Starting Page | 1973 |
| ISSN | 01496395 |
| e-ISSN | 15205754 |
| DOI | 10.1080/01496395.2017.1318921 |
| Journal | Separation Science and Technology |
| Issue Number | 12 |
| Volume Number | 52 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2017-08-13 |
| Access Restriction | Open |
| Subject Keyword | Journal: Separation Science and Technology Analytical Chemistry Adsorption Affinity Chromatography Cryogel Lysozyme Tris |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Filtration and Separation Process Chemistry and Technology |
