NDLI: Preparation of Polyacrylonitrile-Based Immobilized Copper-Ion Affinity Membranes for Protein Adsorption.

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Preparation of Polyacrylonitrile-Based Immobilized Copper-Ion Affinity Membranes for Protein Adsorption.

Content Provider	Europe PMC
Author	Yang, Yin-Jie Chang, Hou-Chien Wang, Min-Ying Suen, Shing-Yi
Editor	Cassano, Alfredo
Copyright Year	2023
Abstract	A polyacrylonitrile (PAN)-based immobilized metal-ion affinity membrane (IMAM) was prepared with a high capacity for protein adsorption. PAN was selected as the substrate due to its excellent thermal and chemical stability. The cyano groups on the PAN membrane were substituted with carboxyl groups, followed by reactions with ethylenediamine (EDA) and ethylene glycol diglycidyl ether (EGDGE) to produce the terminal epoxy groups. The chelating agent iminodiacetic acid (IDA) was then bound to the modified PAN membrane and further chelated with copper ions. The immobilized copper ion amount of membrane was analyzed to obtain the optimal reaction conditions, which were 60 °C/3 h for EDA coupling and 60 °C/4 h for EGDGE grafting. Furthermore, under the use of minor IDA and copper ion concentrations, the immobilized copper ion capacity of the IMAM was 4.8 μmol/cm2 (253.4 µmol/mL, or 1.47 μmol/mg). At a neutral pH, the cationic lysozyme exhibited a large adsorption capacity with the IMAM (1.96 μmol/mL), which was most likely multilayer binding, whereas the adsorption capacity for bovine serum albumin (BSA) and histidine-tagged green fluorescent protein (GFP-His6) was 0.053 μmol/mL and 0.135 μmol/mL, respectively, with a monolayer adsorption arrangement. The protein desorption efficiency was greater than 95%, implying that the prepared IMAM could be reused for protein adsorption.
Volume Number	13
PubMed Central reference number	PMC10056745
Issue Number	3
PubMed reference number	36984658
Journal	Membranes (Basel)
e-ISSN	20770375
DOI	10.3390/membranes13030271
Language	English
Publisher	MDPI
Publisher Date	2023-02-24
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	polyacrylonitrile membrane immobilized metal-ion affinity membrane protein adsorption adsorption arrangement
Content Type	Text
Resource Type	Article
Subject	Chemical Engineering Process Chemistry and Technology Filtration and Separation

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