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Cytokine and inflammatory mediators are associated with cytotoxic, anti-inflammatory and apoptotic activity of honeybee venom
| Content Provider | Scilit |
|---|---|
| Author | Salama, Mohamed A. Younis, Mohamed A. Talaat, Roba M. |
| Copyright Year | 2020 |
| Abstract | Objective The present study aimed to evaluate cytotoxic, apoptotic, and anti-inflammatory properties of bee venom (BV) as well as changes in cytokine secretion levels and nitric oxide (NO) production using three different cancer cell lines [liver (Hep-G2), breast (MCF-7), and cervical (HPV-18 infected HeLa cells)] and two normal cells (splenocytes and macrophages (MQ). Methods Cytotoxic activity of BV against tumor cell lines and normal splenocytes/MQ was tested by MTT assay. By ELISA (ELISA); Tumor necrosis factor (TNF-α), Interleukine (IL-10) and interferon (IFN-γ) were measured. Caspase three expressions was evaluated using reverse transcription-polymerase chain reaction (RT-PCR). Nitric oxide (NO) was estimated using a colorimetric assay. Results BV has a significant cytotoxic effect on all cell lines in a dose- and time-dependent manner; none of them was toxic for normal cells. Treating Hep-G2 cells with BV showed a reduction in IL-10, elevation in TNF-α with no change in IFN-γ level. MCF-7 cells have low IL-10 and TNF-α and high IFN-γ production level. Elevation of IL-10 and IFN-γ coincides with a reduction in TNF-α level was demonstrated in HeLa cells. The expression of Caspase three was dramatically increased with elevation in BV concentration in all tested cancer cell lines. A gradual decrease in NO production by MQ with increasing BV dose was observed. Conclusion Taken together, our results stressed on the importance of BV as a potent anti-tumor agent against various types of cancers (Liver, Breast, and Cervix). Further steps towards the use of BV for pharmacological purposes must be done. |
| Related Links | https://www.degruyter.com/downloadpdf/journals/jcim/ahead-of-print/article-10.1515-jcim-2019-0182/article-10.1515-jcim-2019-0182.pdf |
| ISSN | 15533840 |
| e-ISSN | 15533840 |
| DOI | 10.1515/jcim-2019-0182 |
| Journal | Journal of Complementary and Integrative Medicine |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2020-05-26 |
| Access Restriction | Open |
| Subject Keyword | Journal of Complementary and Integrative Medicine Medical Informatics Cytotoxicity Hep-g2 Hela Inflammation Mcf-7 Journal: Journal of Complementary and Integrative Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Complementary and Alternative Medicine |
