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Kinetic Determination of Serum Glucose by Use of the Hexokinase/Glucose-6-phosphate Dehydrogenase Method
| Content Provider | Scilit |
|---|---|
| Author | Deeg, R. Kraemer, W. Ziegenhorn, J. |
| Copyright Year | 1980 |
| Abstract | We have developed a kinetic fixed-time approach for the quantitative determination of serum glucose by use of the hexokinase/glucose-6-phosphate dehydrogenase method. To achieve a large measuring range, we have apparently increased the Michaelis constant of glucose-6-phosphate dehydrogenase through addition of the competitive inhibitor ATP. By this means, serum samples with glucose concentrations up to 55.5 mmol/l could be analyzed without pre-dilution. The method has been adapted to the ENI GEMSAEC analyzer and to the LKB 2086 Mark II analyzer. It yielded satisfactory results with regard to precision. A comparison of the kinetic procedure with the manual end-point method showed good agreement. No interferences from hemoglobin, bilirubin, or lipemia have been observed. |
| Related Links | https://edoc.hu-berlin.de/bitstream/18452/11057/1/cclm.1980.18.1.49.pdf http://www.degruyter.com/downloadpdf/j/cclm.1980.18.issue-1/cclm.1980.18.1.49/cclm.1980.18.1.49.xml |
| Ending Page | 52 |
| Page Count | 4 |
| Starting Page | 49 |
| ISSN | 14346621 |
| e-ISSN | 14374331 |
| DOI | 10.1515/cclm.1980.18.1.49 |
| Journal | Clinical Chemistry and Laboratory Medicine (CCLM) |
| Issue Number | 1 |
| Volume Number | 18 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 1980-01-01 |
| Access Restriction | Open |
| Subject Keyword | Clinical Chemistry and Laboratory Medicine (cclm) Analytical Chemistry Kinetic Serum Glucose Adapted Phosphate Dehydrogenase Hexokinase/glucose Dehydrogenase Method Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 18 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
