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Determination of glucose-6-phosphate dehydrogenase cut-off values in a Tunisian population
| Content Provider | Scilit |
|---|---|
| Author | Laouini, Naouel Sahli, Chaima Abdelhafidh Jouini, Latifa Haloui, Sabrine Fredj, Sondes Hadj Daboubi, Rym Siala, Hajer Ouali, Faida Becher, Meriam Toumi, Nour ElHouda Bibi, Amina Messsaoud, Taieb |
| Copyright Year | 2017 |
| Abstract | Background:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the commonest enzymopathy worldwide. The incidence depends essentially on the methods used for the assessment. In this respect, we attempted in this study to set cut-off values of G6PD activity to discriminate among normal, heterozygous, and deficient individuals using the World Health Organization (WHO) classification and the receiver operating characteristics (ROC) curve analysis.Methods:Blood samples from 250 female and 302 male subjects were enrolled in this study. The G6PD activity was determined using a quantitative assay. The common G6PD mutations in Tunisia were determined using the amplification refractory mutation system (ARMS-PCR) method. The ROC curve was used to choice the best cut-off.Results:Normal G6PD values were 7.69±2.37, 7.86±2.39, and 7.51±2.35 U/g Hb for the entire, male, and female groups, respectively. Cut-off values for the total, male, and female were determined using the WHO classification and ROC curves analysis. In the male population, both cut-offs established using ROC curve analysis (4.00 U/g Hb) and the 60% level (3.82 U/g Hb), respectively are sensitive and specific resulting in a good efficiency of discrimination between deficient and normal males. For the female group the ROC cut-off (5.84 U/g Hb) seems better than the 60% level cut-off (3.88 U/g Hb) to discriminate between normal and heterozygote or homozygote women with higher Youden Index.Conclusions:The establishment of the normal values for a population is important for a better evaluation of the assay result. The ROC curve analysis is an alternative method to determine the status of patients since it correlates DNA analysis and G6PD activity. |
| Related Links | http://www.degruyter.com/downloadpdf/j/cclm.2017.55.issue-8/cclm-2016-0253/cclm-2016-0253.xml |
| Ending Page | 1201 |
| Page Count | 9 |
| Starting Page | 1193 |
| ISSN | 14346621 |
| e-ISSN | 14374331 |
| DOI | 10.1515/cclm-2016-0253 |
| Journal | Clinical Chemistry and Laboratory Medicine (CCLM) |
| Issue Number | 8 |
| Volume Number | 55 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 2017-01-18 |
| Access Restriction | Open |
| Subject Keyword | Clinical Chemistry and Laboratory Medicine (cclm) Medical Laboratory Technology Cut-off Values G6pd Glucose-6-phosphate Dehydrogenase Molecular Diagnosis Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 55, Issue- 1 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
