NDLI: Non-radioactive Labeling and Detection of Nucleic Acids. II. Optimization of the Digoxigenin System

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Non-radioactive Labeling and Detection of Nucleic Acids. II. Optimization of the Digoxigenin System

Content Provider	Scilit
Author	Höltke, Hans-Joachim Seibl, Rudolf Burg, Josef Mühlegger, Klaus Kessler, Christoph
Copyright Year	1990
Abstract	The random-primed DNA labeling technique was modified for the incorporation of digoxigenin into DNA as basic component of the digoxigenin-based non-radioactive DNA labeling and detection system. Digoxigenin molecules act as reporter groups for highly sensitive DNA detection by a digoxigenin-specific antibody:alkaline phosphatase-conjugate-catalysed color reaction. The parameters affecting the individual reaction steps of the digoxigenin labeling, hybridization and detection reactions were optimized to maximal sensitivity and specificity.
Related Links	https://www.degruyter.com/downloadpdf/journals/bchm/371/2/article-p929.pdf
Ending Page	938
Page Count	10
Starting Page	929
ISSN	01773593
DOI	10.1515/bchm3.1990.371.2.929
Journal	Biological chemistry Hoppe-Seyler
Issue Number	2
Volume Number	371
Language	English
Publisher	Walter de Gruyter GmbH
Publisher Date	1990-01-01
Access Restriction	Open
Subject Keyword	Biological Chemistry Hoppe-seyler Organic Chemistry Antibody Phosphatase Basic Optimized Digoxigenin Maximal Conjugate Dna Labeling Primed Catalysed Journal: Biological chemistry Hoppe-Seyler, Vol- 371
Content Type	Text
Resource Type	Article
Subject	Biochemistry

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