Loading...
Please wait, while we are loading the content...
Similar Documents
Properties of 5-Hydroxyvalerate CoA-Transferase fromClostridium aminovalericum
| Content Provider | Scilit |
|---|---|
| Author | Eikmanns, Ulrich Buckel, Wolfgang |
| Copyright Year | 1990 |
| Abstract | 5-Hydroxyvalerate CoA-transferase from Clostridium aminovalericum, strain T2-7, was purified approximately 100-fold to homogeneity. The molecular mass of the native enzyme was determined by three different methods to be 178 +/- 11 kDa; that of the subunit was 47 kDa, indicating a homotetrameric structure. The following CoA esters acted as substrates (decreasing specificity, V/Km): 5-hydroxyvaleryl-CoA greater than propionyl-CoA greater than acetyl-CoA greater than (Z)-5-hydroxy-2-pentenoyl-CoA greater than butyryl-CoA greater than valeryl-CoA. 4-Pentenoate and 3-pentenoate were also activated by acetyl-CoA to the corresponding CoA esters, whereas crotonate, (E)-5-hydroxy-2-pentenoate, (E)-2-pentenoate and 2,4-pentadienoate were not attacked. 5-Hydroxyvalerate CoA-transferase showed ping-pong kinetics and was inactivated by sodium boranate only in the presence of a CoA substrate. This indicated the formation of a thiolester between a specific carboxyl group of the enzyme and CoASH during the course of the reaction. The CoA-transferase was inhibited by ATP and CTP, slightly by ADP, GTP and UTP, but not by AMP. The inhibition by ATP was competitive towards CoA esters and noncompetitive towards acetate. |
| Related Links | http://www.degruyter.com/downloadpdf/j/bchm3.1990.371.issue-2/bchm3.1990.371.2.1077/bchm3.1990.371.2.1077.xml |
| Ending Page | 1082 |
| Page Count | 6 |
| Starting Page | 1077 |
| ISSN | 01773593 |
| DOI | 10.1515/bchm3.1990.371.2.1077 |
| Journal | Biological chemistry Hoppe-Seyler |
| Issue Number | 2 |
| Volume Number | 371 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 1990-01-01 |
| Access Restriction | Open |
| Subject Keyword | Biological Chemistry Hoppe-seyler Biochemistry and Molecular Biology Coa Transferase Pentenoate Acetyl Kda Hydroxy Atp Aminovalericum Sodium Journal: Biological chemistry Hoppe-Seyler, Vol- 371 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
