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Analysis of Haemoglobin Variants Using Immobilized pH Gradients
| Content Provider | Scilit |
|---|---|
| Author | Sinha, P. Galacteros, F. Righetti, P. G. Kohlmeier, M. Köttgen, E. |
| Copyright Year | 1993 |
| Abstract | We present a new method for analysis of haemoglobin variants in immobilized pH gradients. The isoelectric fractionation is performed in polyacrylamide gels with copolymerized pH gradients between pH 7.0 and 8.0. The common haemoglobin variants (HbA, HbF, HbS, HbC, HbE and HbA2) are clearly resolved within this pH range. The high resolving power and reproducibility of immobilized pH gradients combined with extremely short separation times make this fractionation technique superior to all electrokinetic procedures used for haemoglobin analysis. At present, isoelectric focusing in immobilized pH gradients is a complementary tool for haemoglobin analysis when classical electrophoretic techniques do not provide sufficient resolution. We hope that in the future this technique will gain the popularity it deserves. |
| Related Links | https://edoc.hu-berlin.de/bitstream/18452/13127/1/cclm.1993.31.2.91.pdf http://www.degruyter.com/downloadpdf/j/cclm.1993.31.issue-2/cclm.1993.31.2.91/cclm.1993.31.2.91.xml |
| Ending Page | 96 |
| Page Count | 6 |
| Starting Page | 91 |
| ISSN | 14346621 |
| e-ISSN | 14374331 |
| DOI | 10.1515/cclm.1993.31.2.91 |
| Journal | Clinical Chemistry and Laboratory Medicine (CCLM) |
| Issue Number | 2 |
| Volume Number | 31 |
| Language | English |
| Publisher | Walter de Gruyter GmbH |
| Publisher Date | 1993-01-01 |
| Access Restriction | Open |
| Subject Keyword | Clinical Chemistry and Laboratory Medicine (cclm) Analytical Chemistry Immobilized Ph Gradients Resolving Haemoglobin Variants Haemoglobin Analysis Journal: Clinical Chemistry and Laboratory Medicine (CCLM), Vol- 31 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
