Loading...
Please wait, while we are loading the content...
A Rapid and Efficient Method for the Isolation of Mitochondrial DNA From Wheat Crop
| Content Provider | Scilit |
|---|---|
| Author | Ejaz, Muhammad Gaisheng, Zhang Qidi, Zhu Xinbo, Zhang |
| Copyright Year | 2013 |
| Description | The mitochondrial DNA was isolated from wheat (Triticum aestivum) crop in combination of different centrifugation and density gradient ultracentrifugation, DNaseI enzyme treatment, sucrose sedimentation; lysis with SDS and potassium proteinase. Pure mitochondrial DNA was successfully obtained by phenol/chloroform/isoamyl alcohol extraction to remove protein, and digested with RNase phenol/chloroform extraction method. To detect mitochondrial DNA purity, specific primers were designed for nuclear (Beta-actin) and mitochondrial (COXII) gene. Isolated mitochondrial DNA is pure, suitable for PCR and genetic analysis. |
| ISSN | 19169752 |
| e-ISSN | 19169760 |
| DOI | 10.5539/jas.v5n6p32 |
| Journal | Journal of Agricultural Science |
| Issue Number | 6 |
| Volume Number | 5 |
| Language | English |
| Publisher | Canadian Center of Science and Education |
| Publisher Date | 2013-05-15 |
| Access Restriction | Open |
| Subject Keyword | Biochemical Research Mitochondrial Dna Triticum Aestivum Density Gradient Isolation of Mitochondrial Extraction Method |
| Content Type | Text |
| Resource Type | Article |
