NDLI: Functional endothelial cells derived from rhesus monkey embryonic stem cells

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Functional endothelial cells derived from rhesus monkey embryonic stem cells

Content Provider	Scilit
Author	Kaufman, Dan Lewis, Rachel L. Hanson, Eric T. Auerbach, Robert Plendl, Johanna Thomson, James A.
Copyright Year	2004
Description	We have used rhesus monkey embryonic stem (ES) cells to study endothelial cell development. Rhesus ES cells (R366.4 cell line) exposed to medium containing vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), and epidermal growth factor (EGF) assumed a relatively uniform endothelial cell morphology and could be propagated and expanded with a consistent phenotype and normal karyotype. When placed in Matrigel, these rhesus ES cell–derived endothelial cells (RESDECs) formed capillary-like structures characteristic of endothelial cells. Immunohistochemical and flow cytometric analysis of RESDECs showed that they take up acetylated low-density lipoprotein (LDL), express CD146, von Willebrand factor, and the integrin αvβ3, and bind the lectin ulex europaeus agglutinin-1. These cells also express the VEGF receptor Flk-1 and secrete VEGF. When introduced in a Matrigel plug implanted subcutaneously in mice, RESDECs formed intact vessels and recruited new endothelial cell growth. In vivo function was demonstrated by coinjection of RESDECs with murine tumor cells subcutaneously into immunocompromised adult mice. RESDECs injected alone did not form measurable tumors. Tumor cells grew more rapidly and had increased vascularization when coinjected with the RESDECs. Immunohistochemical staining demonstrated that the RESDECs participated in forming the tumor neovasculature. RESDECs provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies.
Related Links	http://www.bloodjournal.org/content/103/4/1325.full.pdf
Ending Page	1332
Page Count	8
Starting Page	1325
DOI	10.1182/blood-2003-03-0799
Journal	Blood
Issue Number	4
Volume Number	103
Language	English
Publisher	American Society of Hematology
Publisher Date	2004-02-15
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Developmental Biology Lipoprotein Stem Cells Fibroblast Growth Factor Mice Endothelial Cell Vascular Endothelial Growth Factor Insulin Rhesus Monkey Acetylated Journal: Blood (Vol- 78, Issue- 4)
Content Type	Text
Resource Type	Article

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