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Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells
| Content Provider | Scilit |
|---|---|
| Author | Burger, P. E. Coetzee, Sandra McKeehan, Wallace L. Kan, Mikio Cook, Perry Fan, Yong Suda, Toshio Hebbel, Robert P. Novitzky, Nicolas Muller, William A. Wilson, E. Lynette |
| Copyright Year | 2002 |
| Description | Recent experiments show that hematopoietic progenitor cell populations contain endothelial precursor cells. We have isolated a population of $CD34^{+}$ cells that expresses fibroblast growth factor receptor-1 (FGFR-1) and that differentiates into endothelial cells in vitro. We find that 4.5% ± 2.1% of $CD34^{+}$cells isolated from bone marrow, cord blood, and mobilized peripheral blood express FGFR-1 and that viable $CD34^{+}FGFR^{+}$cells are small, with little granularity, and express both primitive hematopoietic and endothelial markers on their surface. The primitive hematopoietic markers AC133, c-kit, and Thy-1 are coexpressed by 75%, 85%, and 64% of $CD34^{+}FGFR^{+}$ cells, respectively. Most of the $CD34^{+}FGFR^{+}$ cells also express antigens found on endothelial cells, such as CD31, vascular endothelial growth factor receptor-2, and the endothelial-specific cell surface marker, vascular endothelial cadherin (VE-cadherin), whereas 56% to 60% of the cells express Tie, Tek, and the endothelial-specific marker, P1H12. The $CD34^{+}FGFR^{+}$ population is enriched in cells expressing endothelial-specific antigens compared with the $CD34^{+}$ population. Isolated $CD34^{+}FGFR^{+}$ cells grow slowly in culture, are stimulated by fibroblast growth factor-2 and vascular endothelial growth factor, and give rise to cells that express von Willebrand factor and VE-cadherin and that incorporate acetylated low-density lipoprotein. These experiments show that FGFR-1 is expressed by a subpopulation of $CD34^{+}$ cells that give rise to endothelial cells in vitro, indicating that this population contains endothelial stem/progenitor cells. |
| Related Links | http://bloodjournal.hematologylibrary.org/content/100/10/3527.full.pdf |
| Ending Page | 3535 |
| Page Count | 9 |
| Starting Page | 3527 |
| DOI | 10.1182/blood.v100.10.3527 |
| Journal | Blood |
| Issue Number | 10 |
| Volume Number | 100 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2002-11-15 |
| Access Restriction | Open |
| Subject Keyword | Research and Experimental Medicine Fibroblast Growth Factor Acetylated Endothelial Cells Vascular Endothelial Growth Factor Lipoprotein Cd34 Bone Marrow Cord Blood Von Willebrand Factor Journal: Blood (Vol- 113, Issue- 10) |
| Content Type | Text |
| Resource Type | Article |
