NDLI: Pharmacokinetics of human granulocyte-macrophage colony-stimulating factor using a sensitive immunoassay

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Pharmacokinetics of human granulocyte-macrophage colony-stimulating factor using a sensitive immunoassay

Content Provider	Scilit
Author	Cebon, J. Dempsey, P. Fox, R. Kannourakis, G. Bonnem, E. Burgess, Aw Morstyn, G.
Copyright Year	1988
Description	A sensitive and reliable sandwich enzyme-linked immunosorbent assay (ELISA) has been developed for recombinant human granulocyte-macrophage colony-stimulating factor (hGM-CSF). The assay is quantitative between 100 pg/mL and 2.5 ng/mL for bacterially synthesized hGM-CSF in human serum and is more sensitive and specific than the semisolid agar bioassay. As part of a phase I study, the pharmacokinetics of intravenous (IV) bolus injection and subcutaneous (SC) administration of hGM-CSF were studied. Following a single IV dose, an initial high blood level of hGM-CSF occurred, followed by a rapid decrease occurring in two apparent phases with a half-life (t1/2)alpha of less than five minutes and a t1/2 beta of 150 minutes. After an SC injection, detectable serum levels occurred within 15 to 30 minutes, and serum levels were sustained for a variable time depending on the dose. At the highest SC dose (10 micrograms/kg), a serum level of greater than 1 ng/mL (65 pmol/L) was maintained for greater than 12 hours after a single injection. This corresponds to the concentration of hGM-CSF supporting near-maximum proliferation in vitro.
Related Links	https://ashpublications.org/blood/article-pdf/72/4/1340/599226/1340.pdf
Ending Page	1347
Page Count	8
Starting Page	1340
DOI	10.1182/blood.v72.4.1340.1340
Journal	Blood
Issue Number	4
Volume Number	72
Language	English
Publisher	American Society of Hematology
Publisher Date	1988-10-01
Access Restriction	Open
Subject Keyword	Medical Laboratory Technology Macrophage Csf Colony Granulocyte T1/2 Hgm Minutes Stimulating Ng/ml Journal: Blood (Vol- 76, Issue- 4)
Content Type	Text
Resource Type	Article

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