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Effects of recombinant human GM-CSF on proliferation of clonogenic cells in acute myeloblastic leukemia
| Content Provider | Scilit |
|---|---|
| Author | Griffin, Jd Young, D. Herrmann, F. Wiper, D. Wagner, K. Sabbath, Kd |
| Copyright Year | 1986 |
| Description | Proliferation of acute myeloblastic leukemia (AML) cells in vitro is limited in most cases to a small subset of blasts that have several properties of stem cells. These leukemic colony-forming cells (AML-CFU) generally require addition of exogenous growth factors for proliferation in agar or methylcellulose. These factors can be supplied by media conditioned by phytohemagglutinin-stimulated normal leukocytes or by CSF-secreting tumor cell lines. However, the exact factor or factors required for stimulation of AML-CFU growth have not been defined. We compared the AML-CFU stimulatory activity of a human recombinant GM-CSF with that of GCT-CM, Mo-CM, and the PHA-leukocyte feeder system in 15 cases of AML. In each of the 12 cases that required exogenous growth factors for maximum AML-CFU growth, recombinant GM-CSF could replace either GM-CSF or Mo-CM, and could partially replace the PHA-leukocyte feeder system. These results indicate that this GM-CSF is a growth promoter of AML-CFU in these culture systems. |
| Related Links | https://ashpublications.org/blood/article-pdf/67/5/1448/593120/1448.pdf |
| Ending Page | 1453 |
| Page Count | 6 |
| Starting Page | 1448 |
| DOI | 10.1182/blood.v67.5.1448.1448 |
| Journal | Blood |
| Issue Number | 5 |
| Volume Number | 67 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1986-05-01 |
| Access Restriction | Open |
| Subject Keyword | Hematology Aml Replace Leukemia Recombinant Cfu Stimulated Gm Csf Myeloblastic Journal: Blood (Vol- 81, Issue- 5) |
| Content Type | Text |
| Resource Type | Article |
