NDLI: Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells

Please wait, while we are loading the content...

Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells

Content Provider	Scilit
Author	Delorme, Bruno Ringe, Jochen Gallay, Nathalie Vern, Yves Le Kerboeuf, Dominique Jorgensen, Christian Rosset, Philippe Sensebé, Luc Layrolle, Pierre Häupl, Thomas Charbord, Pierre
Copyright Year	2008
Description	We have studied the plasma membrane protein phenotype of human culture-amplified and native bone marrow mesenchymal stem cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in colony-forming units–fibroblasts was low for CD49b, CD90, and CD105, but high for CD73, CD130, CD146, CD200, and integrin alphaV/beta5. In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies.
Related Links	http://www.bloodjournal.org/content/111/5/2631.full.pdf
Ending Page	2635
Page Count	5
Starting Page	2631
DOI	10.1182/blood-2007-07-099622
Journal	Blood
Issue Number	5
Volume Number	111
Language	English
Publisher	American Society of Hematology
Publisher Date	2008-03-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Proteins Bone Marrow Hematopoietic Cultured Cells Antigens Mesenchymal Cd73 Amplified Cd146 Fibroblasts Journal: Blood (Vol- 84, Issue- 5)
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in