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Perivascular support of human hematopoietic stem/progenitor cells
| Content Provider | Scilit |
|---|---|
| Author | Corselli, Mirko Chin, Chee Jia Parekh, Chintan Sahaghian, Arineh Wang, Wenyuan Ge, Shundi Evseenko, Denis Wang, Xiaoyan Montelatici, Elisa Lazzari, Lorenza Crooks, Gay M. Péault, Bruno |
| Copyright Year | 2013 |
| Description | Hematopoietic stem and progenitor cells (HSPCs) emerge and develop adjacent to blood vessel walls in the yolk sac, aorta-gonad-mesonephros region, embryonic liver, and fetal bone marrow. In adult mouse bone marrow, perivascular cells shape a “niche” for HSPCs. Mesenchymal stem/stromal cells (MSCs), which support hematopoiesis in culture, are themselves derived in part from perivascular cells. In order to define their direct role in hematopoiesis, we tested the ability of purified human $CD146^{+}$ perivascular cells, as compared with unfractionated MSCs and $CD146^{−}$ cells, to sustain human HSPCs in coculture. $CD146^{+}$ perivascular cells support the long-term persistence, through cell-to-cell contact and at least partly via Notch activation, of human myelolymphoid HSPCs able to engraft primary and secondary immunodeficient mice. Conversely, unfractionated MSCs and $CD146^{−}$ cells induce differentiation and compromise ex vivo maintenance of HSPCs. Moreover, $CD146^{+}$ perivascular cells express, natively and in culture, molecular markers of the vascular hematopoietic niche. Unexpectedly, this dramatic, previously undocumented ability to support hematopoietic stem cells is present in $CD146^{+}$ perivascular cells extracted from the nonhematopoietic adipose tissue. |
| Related Links | https://ashpublications.org/blood/article-pdf/121/15/2891/1365043/2891.pdf |
| Ending Page | 2901 |
| Page Count | 11 |
| Starting Page | 2891 |
| DOI | 10.1182/blood-2012-08-451864 |
| Journal | Blood |
| Issue Number | 15 |
| Volume Number | 121 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2013-04-11 |
| Access Restriction | Open |
| Subject Keyword | Cell Tissue Engineering Hematopoietic Bone Marrow Hspcs Niche Perivascular Hematopoiesis Stem Adult Cd146 Sup Journal: Blood (Vol- 125, Issue- 15) |
| Content Type | Text |
| Resource Type | Article |
