NDLI: Studies with a murine monoclonal antibody that abolishes ristocetin- induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor

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Studies with a murine monoclonal antibody that abolishes ristocetin- induced binding of von Willebrand factor to platelets: additional evidence in support of GPIb as a platelet receptor for von Willebrand factor

Content Provider	Scilit
Author	Coller, Bs Peerschke, Ei Scudder, Le Sullivan, Ca
Copyright Year	1983
Description	A murine monoclonal antibody directed at or near a platelet membrane receptor for the von Willebrand factor was produced by the hybridoma technique. Purified F(ab')2 fragments and/or intact antibody completely blocked the agglutination of platelets induced by both ristocetin and bovine von Willebrand factor and the binding of von Willebrand factor antigen to platelets. The antibody also decreased platelet retention, prevented the reduction in platelet electrophoretic mobility caused by bovine von Willebrand factor, and decreased the serum prothrombin time. Radiolabeled F(ab')2 fragments bound to or approximately 2.5 X 10(4) sites on normal platelets with high affinity (KD or approximately 1.5 X 10(-8) M); there was no binding to platelets from 2 patients with the Bernard-Soulier syndrome. Immunoprecipitation and affinity chromatography studies indicated that the antibody binds to glycoprotein lb at a site contained on the externally oriented portion of the GPIb alpha chain (glycocalicin). An unidentified mol wt or approximately 20,000 molecule labeled by periodate/NaB3H4 coprecipitated and copurified with GPIb.
Related Links	https://ashpublications.org/blood/article-pdf/61/1/99/210909/99.pdf
DOI	10.1182/blood.v61.1.99.bloodjournal61199
Journal	Blood
Issue Number	1
Volume Number	61
Language	English
Publisher	American Society of Hematology
Publisher Date	1983-01-01
Access Restriction	Open
Subject Keyword	Hematology Antibody Binding Von Willebrand Factor Platelets Affinity Murine Journal: Blood (Vol- 3, Issue- 1)
Content Type	Text
Resource Type	Article

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