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The human CD34 hematopoietic stem cell antigen promoter and a 3' enhancer direct hematopoietic expression in tissue culture
| Content Provider | Scilit |
|---|---|
| Author | Burn, Tc Satterthwaite, Ab Tenen, Dg |
| Copyright Year | 1992 |
| Description | The human CD34 hematopoietic stem cell antigen is a highly glycosylated type 1 membrane protein of unknown function. CD34 is expressed on 1% to 4% of bone marrow cells, including pluripotent stem cells and committed progenitors of each hematopoietic lineage. CD34 has also been shown to be expressed on the small vessel endothelium of a variety of tissues and on a subset of bone marrow stromal cells. We have chosen to use the human CD34 gene as model to examine the transcription factors and cis-elements required for stem cell/progenitor cell-specific gene regulation. We show here that the CD34 gene is transcriptionally regulated in tissue culture cells. Using a luciferase reporter gene, we have isolated and characterized an active CD34 promoter. A CD34- luciferase construct, containing 4.5 kb of 5′ flanking DNA from a CD34 genomic clone, was 30-fold more active in CD34+ tissue culture cells than in HeLa cells. Sequences from the 3′ end of the CD34 gene were shown to have enhancing activity in CD34+ T-lymphoblastic RPMI-8402 cells and not in CD34- U937 cells or in nonhematopoietic HeLa cells. We also show that a cytidine-guanosine island in the 5′ end of the CD34 gene is heavily methylated in two CD34- hematopoietic cell lines and demethylated in two CD34+ cell lines. Analysis of the CD34 promoter should result in the identification of stem cell/progenitor cell- specific transcription factors and should provide a means to direct the expression of heterologous genes in hematopoietic stem cells and progenitors. |
| Related Links | https://ashpublications.org/blood/article-pdf/80/12/3051/608867/3051.pdf |
| Ending Page | 3059 |
| Page Count | 9 |
| Starting Page | 3051 |
| DOI | 10.1182/blood.v80.12.3051.3051 |
| Journal | Blood |
| Issue Number | 12 |
| Volume Number | 80 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1992-12-15 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Transcription Factors Hematopoietic Bone Marrow Stem Antigen Cell Specific Culture Cells Cell/progenitor Cell Cd34 Promoter Journal: Blood (Vol- 119, Issue- 12) |
| Content Type | Text |
| Resource Type | Article |
