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Accelerated apoptosis in peripheral blood mononuclear cells (PBMCs) from human immunodeficiency virus type-1 infected patients and in CD4 cross-linked PBMCs from normal individuals
| Content Provider | Scilit |
|---|---|
| Author | Oyaizu, N. McCloskey, Tw Coronesi, M. Chirmule, N. Kalyanaraman, Vs Pahwa, S. |
| Copyright Year | 1993 |
| Description | This study investigates apoptosis as a mechanism for CD4+ T-cell depletion in human immunodeficiency virus type-1 (HIV-1) infection. Although several recent studies have shown that T cells of HIV-infected individuals show enhanced susceptibility to cell death by apoptosis, the mechanisms responsible for apoptosis are largely unknown. By using a flow cytometric technique and by morphology, we have quantitated the percentage of cells undergoing apoptosis in peripheral blood mononuclear cells (PBMCs) from HIV-seronegative donors and from HIV- infected asymptomatic patients. The PBMCs were cultured without any stimulus or with staphylococcus enterotoxin B, anti-T-cell receptor (TCR) alpha beta monoclonal antibody WT-31, or phytohemagglutinin for periods up to 6 days. In addition, we sought to determine whether cross- linking of CD4 followed by various modes of TCR stimulation in vitro could induce apoptosis in normal PBMCs. Here we show that (1) patient PMBCs undergo marked spontaneous apoptosis; (2) stimulation of T cells of patients as well as normal donors results in increased apoptosis; and (3) cross-linking of CD4 molecules is sufficient to induce apoptosis in CD4+ T cells if cross-linking is performed in unfractioned PBMCs, but not if CD4 molecules are cross-linked in purified T-cell preparations. These observations strongly suggest that accelerated cell death through apoptosis plays an important role in the pathogenesis of HIV-1 infection. At the same time, our observations implicate cross- linking of CD4 in vivo as a major contributor to this mechanism of accelerated cell death in HIV infection. |
| Related Links | https://ashpublications.org/blood/article-pdf/82/11/3392/610918/3392.pdf |
| Ending Page | 3400 |
| Page Count | 9 |
| Starting Page | 3392 |
| DOI | 10.1182/blood.v82.11.3392.3392 |
| Journal | Blood |
| Issue Number | 11 |
| Volume Number | 82 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1993-12-01 |
| Access Restriction | Open |
| Subject Keyword | Virology Cell Death Hiv Cd4 Cross Linking Undergoing Apoptosis in Peripheral Pbmcs From Normal Journal: Blood (Vol- 116, Issue- 11) |
| Content Type | Text |
| Resource Type | Article |
