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Involvement of E-selectin in recruitment of endothelial progenitor cells and angiogenesis in ischemic muscle
| Content Provider | Scilit |
|---|---|
| Author | Oh, Il-Young Yoon, Chang-Hwan Hur, Jin Kim, Ji-Hyun Kim, Tae-Youn Lee, Choon-Soo Park, Kyung-Woo Chae, I. -H. Oh, Byung-Hee Park, Young-Bae Kim, Hyo-Soo |
| Copyright Year | 2007 |
| Description | E-selectin plays critical roles in tethering leukocytes to endothelial cells (ECs). We studied the role of E-selectin in endothelial progenitor cell (EPC) homing and vasculogenesis. After ischemia, the expression of E-selectin on ECs peaked 6 to 12 hours and returned to baseline at 24 hours, whereas the level of soluble E-selectin (sE-selectin) in serum increased over 24 hours and remained high at day 7. Mouse bone marrow–derived EPCs expressed not only E-selectin but also its ligand. Homing of circulating EPCs to ischemic limb was significantly impaired in E-selectin knock-out mice, as well as wild-type mice pretreated with blocking antibody against E-selectin, which was rescued by local sE-selectin injection. Mechanism for this is that sE-selectin stimulated not only ECs to express ICAM-1, but also EPCs to secrete interleukin-8 (IL-8), leading to enhanced migration and incorporation to ECs capillary formation. In therapeutic aspect, local treatment with sE-selectin enhanced efficacy of EPC transplantation for vasculogenesis and salvage of ischemic limb. Conversely, when E-selectin was knocked down by E-selectin small interfering RNA, blood flow recovery after EPC transplantation was significantly impaired. But this impaired vasculogenesis was rescued by sE-selectin. In conclusion, these data demonstrate E-selectin is a pivotal molecule for EPCs' homing to ischemic limb and vasculogenesis. |
| Related Links | http://www.bloodjournal.org/content/bloodjournal/110/12/3891.full.pdf |
| Ending Page | 3899 |
| Page Count | 9 |
| Starting Page | 3891 |
| DOI | 10.1182/blood-2006-10-048991 |
| Journal | Blood |
| Issue Number | 12 |
| Volume Number | 110 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2007-12-01 |
| Access Restriction | Open |
| Subject Keyword | Research and Experimental Medicine Endothelial Progenitor Cell Selectin Angiogenesis Small Interfering Rna Leukocytes Vasculogenesis Ecs Epcs Salvage Journal: Blood (Vol- 121, Issue- 12) |
| Content Type | Text |
| Resource Type | Article |
