Loading...
Please wait, while we are loading the content...
Similar Documents
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor
| Content Provider | Scilit |
|---|---|
| Author | Berthou, C. Marolleau, Jp Lafaurie, C. Soulie, A. Cortivo, L. Dal Bourge, Jf Benbunan, M. Sasportes, M. |
| Copyright Year | 1995 |
| Description | Granzyme B and perforin are cytoplasmic granule-associated proteins used by cytotoxic T lymphocytes and natural killer (NK) cells to kill their targets. However, granzyme B gene expression has also been detected in a non-cytotoxic hematopoietic murine multipotent stem cell line, FDCP-Mix. The objective of the present study was to investigate whether granzyme B and perforin could be expressed in human hematopoietic CD34+ cells and if present, discover what their physiologic relevance could be. The primitive CD34+ human cell line KG1a was investigated first and was found to express granzyme B and perforin. Highly purified hematopoietic stem/progenitor cells were then selected using the CD34 surface antigen as marker. Steady-state bone marrow (BM) CD34+ cells did not contain these proteins. Peripheral blood (PB) CD34+ cells, which had been induced to circulate, were also analyzed. After chemotherapy (CT) and granulocyte colony-stimulating factor (G-CSF) treatment, CD34+ cells strongly expressed mRNAs and proteins of granzyme B and perforin. In contrast, CD34+ cells mobilized by G-CSF alone were negative. Western blot analysis further showed that granzyme B and perforin proteins were identical in CD34+ cells and activated PBLs. Such proteins might be implicated in the highly efficient migration of CD34+ stem/progenitor cells from BM to PB after CT and G-CSF treatment. The cellular adhesion mechanisms involved in the BM homing of CD34+ cells are disrupted at least temporarily after CT. The Asp-ase proteolytic activity of granzyme B on extracellular matrix proteins could be used by progenitor cells for their rapid detachment from BM stromal cells and perforin might facilitate their migration across the endothelial cell barrier. |
| Related Links | https://ashpublications.org/blood/article-pdf/86/9/3500/618040/3500.pdf |
| Ending Page | 3506 |
| Page Count | 7 |
| Starting Page | 3500 |
| DOI | 10.1182/blood.v86.9.3500.bloodjournal8693500 |
| Journal | Blood |
| Issue Number | 9 |
| Volume Number | 86 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1995-11-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Gene Expression Hematopoietic Bone Marrow Proteins Cd34 Stem/progenitor Cells Granulocyte Colony Granzyme B and Perforin Journal: Blood (Vol- 108, Issue- 9) |
| Content Type | Text |
| Resource Type | Article |
