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Durable engraftment of AMD3100-mobilized autologous and allogeneic peripheral-blood mononuclear cells in a canine transplantation model
| Content Provider | Scilit |
|---|---|
| Author | Burroughs, Lauri Mielcarek, Marco Little, Marie-Térèse Bridger, Gary Farland, Ron Mac Fricker, Simon Labrecque, Jean Sandmaier, Brenda M. Storb, Rainer |
| Copyright Year | 2005 |
| Description | Peripheral-blood mononuclear cells (PBMCs) mobilized with AMD3100, a CXCR4 antagonist, combined with granulocyte colony-stimulating factor (G-CSF) have reconstituted autologous hematopoiesis in cancer patients following myeloablative conditioning. The engraftment potential of PBMCs mobilized with AMD3100 alone, however, has remained unproven. We therefore studied AMD3100-mobilized PBMCs in a canine model. Four dogs received 920 cGy total body irradiation (TBI) before infusion of autologous AMD3100-mobilized PBMCs (median CD34 cell count, 3.9 × 106/kg). Neutrophil (> 0.5 × 109/L [500/μL]) and platelet (> 20 ×/109/L [> 20 000/μL]) recoveries occurred at medians of 9 (range, 7-10) days and 25 (range, 23-38) days, respectively, after TBI, and all dogs had normal marrow function at 1 year after transplantation. To evaluate the long-term engraftment potential of AMD3100-mobilized PBMCs, 5 dogs were given 920 cGy TBI followed by infusion of AMD3100-mobilized PBMCs (median CD34 cell dose, 4.7 × 106/kg) from their dog leukocyte antigen (DLA)-identical littermates. Neutrophil and platelet recoveries occurred at medians of 8 (range, 8-10) days and 26 (range, 26-37) days, respectively, after TBI. With a median follow-up of 53 (range, 33-61) weeks, recipients' marrow function was normal, and blood-donor chimerism levels were 97% to 100%. In summary, both autologous and allogeneic AMD3100-mobilized PBMCs led to prompt and durable engraftment in dogs after 920 cGy TBI. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1895108/pdf |
| Ending Page | 4008 |
| Page Count | 7 |
| Starting Page | 4002 |
| DOI | 10.1182/blood-2005-05-1937 |
| Journal | Blood |
| Issue Number | 12 |
| Volume Number | 106 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2005-12-01 |
| Access Restriction | Open |
| Subject Keyword | Veterinary Sciences Peripheral Blood Mononuclear Cell Journal: Blood (Vol- 120, Issue- 12) |
| Content Type | Text |
| Resource Type | Article |
