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CpG methylation of the major Epstein-Barr virus latency promoter in Burkitt's lymphoma and Hodgkin's disease
| Content Provider | Scilit |
|---|---|
| Author | Robertson, Kd Manns, A. Swinnen, Lj Zong, Jc Gulley, Ml Ambinder, Rf |
| Copyright Year | 1996 |
| Description | The Epstein-Barr virus (EBV) latency C promoter drives expression of a family of viral proteins commonly targeted by CD8 cytotoxic T cells. These proteins are not generally expressed in African Burkitt's lymphoma and in EBV-associated Hodgkin's disease. The failure to express these proteins is almost certainly an important factor in the evasion of immunosurveillance by EBV-associated tumors. In a previous study, we have shown that transcriptional activation of the C promoter is inhibited by methylation of a particular CpG site upstream of the promoter that prevents binding of a cellular protein (CBF2), and we have shown that this and adjacent CpG sites are methylated in a Burkitt's lymphoma cell line. In the present study, we show that CpG sites in the CBF2 binding region are predominantly methylated in African Burkitt's lymphoma and in EBV-associated Hodgkin's disease. In addition, we present the first direct evidence that the C promoter is transcriptionally silent in Burkitt's lymphoma. In contrast, we show a complete absence of methylation in the CBF2 binding region in a case of reversible EBV-associated B-cell lymphoma arising in an immunocompromised patient whose tumor shows C promoter transcriptional activity. By inhibiting expression of highly antigenic viral proteins, methylation of transcriptional control sequences may veil the presence of virus in tumor tissue from CD8(+) cytotoxic T-cell immune surveillance and thus facilitate viral tumorigenesis. |
| Related Links | https://ashpublications.org/blood/article-pdf/88/8/3129/623211/3129.pdf |
| Ending Page | 3136 |
| Page Count | 8 |
| Starting Page | 3129 |
| DOI | 10.1182/blood.v88.8.3129.bloodjournal8883129 |
| Journal | Blood |
| Issue Number | 8 |
| Volume Number | 88 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1996-10-15 |
| Access Restriction | Open |
| Subject Keyword | Virology Virus Viral Proteins Methylation Ebv Burkitt's Lymphoma Lymphoma Cell Associated Hodgkin's Journal: Blood (Vol- 103, Issue- 8) |
| Content Type | Text |
| Resource Type | Article |
