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CD1d-Restricted NKT Cells Contribute to the Age-Associated Decline of T Cell Immunity
| Content Provider | Scilit |
|---|---|
| Author | Faunce, Douglas E. Palmer, Jessica L. Paskowicz, Kelly K. Witte, Pamela L. Kovacs, Elizabeth J. |
| Copyright Year | 2005 |
| Description | NKT cells are known to regulate effector T cell immunity during tolerance, autoimmunity, and antitumor immunity. Whether age-related changes in NKT cell number or function occur remains unclear. Here, we investigated whether young vs aged (3 vs 22 mo old) mice had different numbers of CD1d-restricted NKT cells and whether activation of NKT cells by CD1d in vivo contributed to age-related suppression of T cell immunity. Flow cytometric analyses of spleen and LN cells revealed a 2- to 3-fold increase in the number of CD1d tetramer-positive NKT cells in aged mice. To determine whether NKT cells from aged mice differentially regulated T cell immunity, we first examined whether depletion of NK/NKT cells affected the proliferative capacity of splenic T cells. Compared with those from young mice, intact T cell preparations from aged mice had impaired proliferative responses whereas NK/NKT-depleted preparations did not. To examine the specific contribution of NKT cells to age-related T cell dysfunction, Ag-specific delayed-type hypersensitivity and T cell proliferation were examined in young vs aged mice given anti-CD1d mAb systemically. Compared with young mice, aged mice given control IgG exhibited impaired Ag-specific delayed-type hypersensitivity and T cell proliferation, which could be significantly prevented by systemic anti-CD1d mAb treatment. The age-related impairments in T cell immunity correlated with an increase in the production of the immunosuppressive cytokine IL-10 by splenocytes that was likewise prevented by anti-CD1d mAb treatment. Together, our results suggest that CD1d activation of NKT cells contributes to suppression of effector T cell immunity in aged mice. |
| Ending Page | 3109 |
| Page Count | 8 |
| Starting Page | 3102 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.175.5.3102 |
| Journal | The Journal of Immunology |
| Issue Number | 5 |
| Volume Number | 175 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2005-09-01 |
| Access Restriction | Open |
| Subject Keyword | Nkt Cells Aged Mice Age Related Cell Immunity Cells Contribute Effector T Cell Activation of Nkt Young Vs Aged |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
