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Augmentation in Expression of Activation-Induced Genes Differentiates Memory from Naive CD4+ T Cells and Is a Molecular Mechanism for Enhanced Cellular Response of Memory CD4+ T Cells
| Content Provider | Scilit |
|---|---|
| Author | Liu, Kebin Li, Yu Prabhu, Vinayakumar Young, Lynn Becker, Kevin G. Munson, Peter J. Weng, Nan-Ping |
| Copyright Year | 2001 |
| Description | In an attempt to understand the molecular basis for the immunological memory response, we have used cDNA microarrays to measure gene expression of human memory and naive $CD4^{+}$ T cells at rest and after activation. Our analysis of 54,768 cDNA clones provides the first glimpse into gene expression patterns of memory and naive $CD4^{+}$ T cells at the genome-scale and reveals several novel findings. First, memory and naive $CD4^{+}$ T cells expressed similar numbers of genes at rest and after activation. Second, we have identified 14 cDNA clones that expressed higher levels of transcripts in memory cells than in naive cells. Third, we have identified 135 (130 known genes and 5 expressed sequence tags) up-regulated and 68 (42 known genes and 26 expressed sequence tags) down-regulated cDNA clones in memory $CD4^{+}$ T after in vitro stimulation with anti-CD3 plus anti-CD28. Interestingly, the increase in mRNA levels of up-regulated genes was greater in memory than in naive $CD4^{+}$ T cells after in vitro stimulation and was higher with anti-CD3 plus anti-CD28 than with anti-CD3 alone in both memory and naive $CD4^{+}$ T cells. Finally, the changes in expression of actin and cytokine genes identified by cDNA microarrays were confirmed by Northern and protein analyses. Together, we have identified ∼200 cDNA clones whose expression levels changed after activation and suggest that the level of expression of up-regulated genes is a molecular mechanism that differentiates the response of memory from naive $CD4^{+}$ T cells. |
| Ending Page | 7344 |
| Page Count | 10 |
| Starting Page | 7335 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.166.12.7335 |
| Journal | The Journal of Immunology |
| Issue Number | 12 |
| Volume Number | 166 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2001-06-15 |
| Access Restriction | Open |
| Subject Keyword | Gene Expression Cdna Microarrays Naive Cd4 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
