NDLI: APC protein immunoexpression in colorectal adenoma and adenocarcinoma

Content Provider	Scientific Electronic Library Online (SciELO)
Author	Bourroul, Vivian Sati Oba Bourroul, Guilherme Muniz Toloi, Giovanna Canato Palma, Rogério Tadeu Oshima, Celina Tizuko Fujiyama Gomes, Thiago Simão Ihara, Sílvia Saiuli Miki Waisberg, Jaques
Abstract	BACKGROUND: activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma. OBJECTIVE: detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa. METHODS: 42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained. RESULTS: the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24). CONCLUSIONS: the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism.
Related Links	http://www.scielo.br/scielo.php?script=sci_abstract&pid=S2237-93632013000300118&lng=en&nrm=iso http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632013000300118&lng=en&nrm=iso
Starting Page	118
Ending Page	125
Page Count	8
File Format	PDF
ISSN	22379363
DOI	10.1016/j.jcol.2013.06.002
Journal	Journal of Coloproctology (Rio de Janeiro)
Issue Number	3
Volume Number	33
Language	English
Publisher	Sociedade Brasileira de Coloproctologia
Publisher Date	2013-09-01
Publisher Institution	Hospital do Servidor Público Estadual Service of Digestive System Surgery Hospital Estadual Mário Covas Service of Digestive System Surgery Faculdade de Medicina do ABC Universidade Federal de São Paulo Escola Paulista de Medicina Laboratory of Molecular Pathology UNIFESP EPM Laboratory of Molecular Pathology Faculdade de Medicina do ABC General and Digestive System Surgery
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Colorectal neoplasms Carcinoma Adenoma APC Gene Immunohistochemistry Biological tumor markers Neoplasias colorretais Gene APC Imunoistoquímica Marcadores biológicos de tumor
Alternative Title	Imunoexpressão da proteína APC nos tecidos de adenoma e de adenocarcinoma colorretais
Content Type	Text
Resource Type	Article
Subject	Gastroenterology

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