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The Long Intergenic Noncoding RNA 00707 Sponges MicroRNA-613 (miR-613) to Promote Proliferation and Invasion of Gliomas
| Content Provider | SAGE Publishing |
|---|---|
| Author | Liu, Handong Hu, Keqi |
| Copyright Year | 2020 |
| Abstract | Glioma is one of the most deadly malignant tumors in humans. Long non-coding RNA (lncRNA) plays a key role in the occurrence, development and invasion of tumors by regulating oncogenic and tumor suppressor pathways. However, the role and action mechanism of long intergenic non-coding RNA 00707 (LINC00707) in gliomas have not been elucidated. This study aimed to investigate the interaction between LINC00707 and miR-613 as well as its role in gliomas.Materials and Methods:The expression levels of LINC00707 and miR-613 were detected by qRT-PCR. The chi-square test was used to analyze the correlation between LINC00707 expression and clinicopathological parameters. CCK-8 and colony formation assays were used to detect glioma cell proliferation; and wound healing and transwell assays were used to detect glioma cell migration and invasion. The relationship between LINC00707 and miR-613 was predicted by Starbase, and verified by qRT-PCR and dual luciferase reporter gene assay.Results:LINC00707 was up-regulated in gliomas. Up-regulated LINC00707 increased the proliferation, migration and invasion of glioma cells, and silenced LINC00707 reduced these abilities. The increase of the expression level of LINC00707 down-regulated miR-613 in glioma cells, while the inhibition of the expression level of LINC00707 up-regulated miR-613 in glioma cells. The high expression of LINC00707 was related to the Karnofsky performance status (KPS) score and WHO staging. LINC00707 could offset the ability of miR-613 to inhibit glioma proliferation and invasion.Conclusion:LINC00707 promotes proliferation and invasion of glioma cells by sponging miR-613. The regulatory axis of LINC00707/miR-613 provides new insights into the mechanism and treatment of gliomas. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/1533033820962092?download=true |
| ISSN | 15330346 |
| Volume Number | 19 |
| Journal | Technology in Cancer Research & Treatment (TCT) |
| e-ISSN | 15330338 |
| DOI | 10.1177/1533033820962092 |
| Language | English |
| Publisher | Sage Publications CA |
| Publisher Date | 2020-10-27 |
| Publisher Place | Los Angeles |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2020 |
| Subject Keyword | LINC00707 glioma invasion miR-613 proliferation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
