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Indoleamine 2,3-Dioxygenase-1 Expression is Changed During Bladder Cancer Cell Invasion
| Content Provider | SAGE Publishing |
|---|---|
| Author | Hellen Joyce Sousa Pereira Santos Luiz Henrique Gomes Matheus Silva, Aline Dalmazzo, Stephanie Vanin Santos, Andressa Assunção Letícia Rafaela Alves Rubens Santos Souza, Diego Mota Reis, Sabrina Thalita Nascimento, Ivan Pereira Dellê, Humberto |
| Copyright Year | 2022 |
| Abstract | The severity of the bladder carcinoma (BC) is directly linked to cell invasion and metastasis. Indoleamine 2,3-dioxygenase-1 (IDO-1) is an INF-γ-induced immunomodulating enzyme that has been linked to the cancer cell invasiveness. Because IDO1 is variable among the tumors, we analyzed its expression in the BC invasion using BC mice models and cell culture. MB49 cells were orthotopically or ectopically inoculated in C57Bl6 mice to evaluate IDO1 by immunohistochemistry. For in vitro experiments, expression of IDO1 and INF-γ was evaluated in grade-1 (RT4) and in grade-3 (T24) BC cell lines. Invading and non-invading T24 cells were separated using the Matrigel/Transwell system, of which total RNA was extracted immediately or after 2 weeks of subculture. Finally, IDO1 was silenced in T24 cells to verify its role on cell invasiveness. In both animal models, IDO1 was differentially expressed between non-invading and invading cells. In cell culture, T24 cells expressed more IDO1 than RT4 cells, independently of the INF-γ expression. IDO1 was differentially expressed between non-invading and invading T24 cells, a difference that was lost by long-time subculture. IDO1 silencing resulted in diminished cell invasiveness. In conclusion, IDO1 expression is changed during bladder carcinoma invasion, playing an important role in this process. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/11786469211065612?download=true |
| ISSN | 11786469 |
| Volume Number | 15 |
| Journal | International Journal of Tryptophan Research (TRY) |
| e-ISSN | 11786469 |
| DOI | 10.1177/11786469211065612 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2022-01-04 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2022 |
| Subject Keyword | indoleamine 2,3-dioxygenase cancer invasiveness Bladder cancer metastasis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Biochemistry |
