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Comparative evaluation of three methylene dianiline isomers in the bacterial reverse mutation assay, the in vitro gene mutation test, and the in vitro chromosomal aberration test
| Content Provider | SAGE Publishing |
|---|---|
| Author | Unterberger-Henig, Elif |
| Copyright Year | 2022 |
| Abstract | 4,4′-MDA is classified as a genotoxic carcinogen based on numerous in vitro and animal data. The consequential assumption that a safe threshold does not exist is not only applied to 4,4′-MDA but also to its structural isomers and impurities 2,2′- and 2,4′-MDA in the absence of substance-specific data. This constitutes a problem in human risk assessments for all three substances as the inherent risks of 2,2′- and 2,4′-MDA and their contribution as impurities to that of 4,4′-MDA are essentially unknown. A comparative in vitro genotoxicity dataset consisting of the bacterial reverse mutation (Ames) test and the chromosomal aberration test in human lymphocytes (both performed according to the current OECD Guidelines) was generated for all three isomers. Furthermore, an in vitro gene mutation test in Chinese hamster ovary (CHO) cells (HPRT locus assay) was conducted with 2,4′-MDA. The results indicate differences regarding the genotoxic mechanism and potential, respectively, between the three structures and suggest that the no-threshold assumption for 4,4′-MDA may not be appropriate for 2,2′- and 2,4′-MDA. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/0748233221091018?download=true |
| Starting Page | 529 |
| Ending Page | 543 |
| Page Count | 15 |
| ISSN | 07482337 |
| Issue Number | 9 |
| Volume Number | 38 |
| Journal | Toxicology and Industrial Health (TIH) |
| e-ISSN | 14770393 |
| DOI | 10.1177/0748233221091018 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2022-05-10 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2022 |
| Subject Keyword | reverse bacterial mutation (Ames) test chromosomal aberration test 4,4′-MDA, 2,2′-MDA, 2,4′-MDA in vitro genotoxicity HPRT locus assay Methylenedianiline risk assessment |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health Toxicology |
