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miR-199a-5p inhibits proliferation and induces apoptosis in hemangioma cells through targeting HIF1A
| Content Provider | SAGE Publishing |
|---|---|
| Author | Wang, Yang Dai, Yu-Xin Wang, Shu-Qing Qiu, Ming-Ke Quan, Zhi-Wei Liu, Ying-Bin Ou, Jing-Min |
| Copyright Year | 2017 |
| Abstract | MicroRNAs (miRNAs) exhibit a crucial role in the regulation of angiogenesis and tumor progression, of which miR-199a-5p (miR-199a) has been reported to function as a tumor suppressor in multiple malignancies. However, the precise mechanisms underlying miR-199a in hemangiomas (HAs) remain elusive. In this study, we found that miR-199a had low expression level, while proliferating cell nuclear antigen (PCNA) had high expression level in proliferating-phase HAs compared with the involuting-phase HAs and normal tissues. Spearman correlation analysis revealed the negative correlation of miR-199a with PCNA expression in proliferating-phase HAs. In vitro experiments showed that restoration of miR-199a suppressed cell proliferation capability and induced cell apoptosis in HA-derived endothelial cells (HDEC) and CRL-2586 EOMA cells, followed with decreased PCNA expression and increased cleaved caspase-3 expression, but miR-199a inhibitor reversed these effects. Furthermore, HIF1A was identified as a target of miR-199a and had negative correlation with miR-199a expression in proliferating-phase HAs. Overexpression of HIF1A attenuated the anti-proliferation effect of miR-199a mimic in HAs cells. Taken together, our findings demonstrate that miR-199a may inhibit proliferation and induce apoptosis in HAs cells via targeting HIF1A and provide a potential therapeutic target for HAs. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/0394632017749357?download=true |
| ISSN | 03946320 |
| Volume Number | 31 |
| Journal | International Journal of Immunopathology and Pharmacology (IJI) |
| e-ISSN | 20587384 |
| DOI | 10.1177/0394632017749357 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2017-12-22 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2017 |
| Subject Keyword | apoptosis miR-199a-5p proliferation hemangioma HIF1A |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Pharmacology Immunology |
