Loading...
Please wait, while we are loading the content...
Similar Documents
rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis isolates from rural areas of Zhejiang, China
| Content Provider | SAGE Publishing |
|---|---|
| Author | Zeng, Mei-Chun Jia, Qing-Jun Tang, Lei-Ming |
| Copyright Year | 2021 |
| Abstract | ObjectiveThe aim was to analyze genetic mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis isolates (RIFR-MTB) from Zhejiang, China.MethodsWe prospectively analyzed RIFR-associated mutations in 13 rural areas of Zhejiang. Isolates were subjected to species identification, phenotype drug susceptibility testing (DST), DNA extraction, and rpoB gene sequencing.ResultsA total of 103 RIFR isolates were identified by DST (22 RIFR only, 14 poly-drug resistant, 49 multidrug resistant, 13 pre-extensively drug resistant [pre-XDR], and 5 extensively drug resistant [XDR]) from 2152 culture-positive sputum specimens. Gene sequencing of rpoB showed that the most frequent mutation was S450L (37.86%, 39/103); mutations P280L, E521K, and D595Y were outside the rifampicin resistance-determining region (RRDR) but may be associated with RIFR. Mutations associated with poly-drug resistant, pre-XDR, and XDR TB were mainly located at codon 445 or 450 in the RRDR.ConclusionsThe frequency of rpoB RRDR mutation in Zhejiang is high. Further studies are needed to clarify the relationships between RIFR and the TTC insertion at codon 433 in the RRDR and the P280L and D595Y mutations outside the RRDR. |
| Related Links | https://journals.sagepub.com/doi/pdf/10.1177/0300060521997596?download=true |
| ISSN | 03000605 |
| Issue Number | 3 |
| Volume Number | 49 |
| Journal | Journal of International Medical Research (IMR) |
| e-ISSN | 14732300 |
| DOI | 10.1177/0300060521997596 |
| Language | English |
| Publisher | Sage Publications UK |
| Publisher Date | 2021-03-09 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights Holder | © The Author(s) 2021 |
| Subject Keyword | multidrug resistance Mycobacterium tuberculosis Tuberculosis rpoB gene mutation rifampin resistance-determining region rifampin resistance |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Cell Biology Biochemistry |
