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After trypsinisation, 3D spheroids of C3A hepatocytes need 18 days to re-establish similar levels of key physiological functions to those seen in the liver

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Toxicology Research Vol - 2 Issue - 1

Toxicology Research Vol -1

After trypsinisation, 3D spheroids of C3A hepatocytes need 18 days to re-establish similar levels of key physiological functions to those seen in the liver

Content Provider	Royal Society of Chemistry (RSC)
Author	Wrzesinski, Krzysztof Fey, Stephen J.
Copyright Year	2013
Abstract	The introduction of trypsinisation in the 1950's was a paradigm shift which helped instigate cell culture. We demonstrate here that human hepatocyte cell line HepG2–C3A needs at least 18 days after trypsinisation to re-establish key ultrastructural and physiological traits. After trypsinisation, cells start to recover these traits at similar rates in both monolayer (2D) or spheroid (3D) growth environments. While this development is restarted by trypsinisation of 2D cultures (typically after 5 days), recovery continues in 3D cultures up until 15–18 days when changes in growth rate, adenylate kinase, ATP, urea and cholesterol all suggest that spheroids undergo some type of physiological transition. Several other cell lines (e.g. Caco-2, HT 29, MDCK, MCF-10A and HepG2 used to model the small and large intestine, kidney, breast acini and liver respectively) are reported in the literature to exhibit very similar changes, on a similar timescale to those reported here. These changes may thus represent a ubiquitous recovery process after trypsinisation rather than differentiation. This would partially explain the common observation that cells grown in 3D exhibit physiological capabilities that are closer to those seen in the intact tissue or organ.
Starting Page	123
Ending Page	135
Page Count	13
File Format	HTM / HTML PDF
ISSN	2045452X
Volume Number	2
Issue Number	2
Journal	Toxicology Research
DOI	10.1039/c2tx20060k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cell culture Liver Spheroid Acinus Cholesterol Kidney Urea Hepatocyte Adenylate kinase Caco-2 Large intestine ATP Adenosine triphosphate
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology

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