NDLI: Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells

Please wait, while we are loading the content...

A low toxic synthetic dendrimer conjugated podophyllotoxin nanodevice with potent antitumor activity against the DMBA/TPA induced mouse skin carcinogenesis model

p,p′-Dichlorodiphenyltrichloroethane inhibits the apoptosis of colorectal adenocarcinoma DLD1 cells through PI3K/AKT and Hedgehog/Gli1 signaling pathways

Respiratory exposure to single-walled carbon nanotubes induced changes in vascular homeostasis and the expression of peripheral blood related genes in a rat model

Prenatal ethanol exposure induces an intrauterine programming of enhanced sensitivity of the hypothalamic–pituitary–adrenal axis in female offspring rats fed with post-weaning high-fat diet

In vitro effects of low-level aldehyde exposures on human umbilical vein endothelial cells

The role of breast cancer resistance protein (Bcrp/Abcg2) in triptolide-induced testis toxicity

Vitamin E ameliorates neurodegeneration related phenotypes caused by neurotoxicity of Al₂O₃-nanoparticles in C. elegans

Endosulfan inducing blood hypercoagulability and endothelial cells apoptosis via the death receptor pathway in Wistar rats

In vitro toxicological characterisation of arsenic-containing fatty acids and three of their metabolites

Development and use of in vitro alternatives to animal testing by the pharmaceutical industry 1980–2013

Umbelliferone β-d-galactopyranoside inhibits chemically induced renal carcinogenesis via alteration of oxidative stress, hyperproliferation and inflammation: possible role of NF-κB

Amelioration of mercury nephrotoxicity after pharmacological manipulation of organic anion transporter 1 (Oat1) and multidrug resistance-associated protein 2 (Mrp2) with furosemide

Non-clinical development of ozanezumab: a humanised antibody targeting the amino terminus of neurite outgrowth inhibitor A (Nogo-A)

Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells

CTAB induced mitochondrial apoptosis by activating the AMPK–p53 pathway in hepatocarcinoma cells

Pathological changes and effect on the learning and memory ability in rats exposed to fluoride and aluminum

Insight into biological system responses in goldfish (Carassius auratus) to multiple doses of avermectin exposure by integrated ¹H NMR-based metabolomics

1,2-Dichloroethane induced nephrotoxicity through ROS mediated apoptosis in vitro and in vivo

Inflammatory cytokine COX-2 mediated cell proliferation through increasing cyclin D1 expression induced by inorganic arsenic in SV-HUC-1 human uroepithelial cells

An experimental and theoretical assessment of quantum dot cytotoxicity

Synthesis and in vivo toxicity assessment of CdSe:ZnS quantum dots functionalized with EDTA-Bis-Cysteamine

Correction: Differential effects of p38 and JNK activation by GSK3 on cadmium-induced autophagy and apoptosis

Correction: Umbelliferone β-d-galactopyranoside exerts an anti-inflammatory effect by attenuating COX-1 and COX-2

Back cover

Toxicology Research Vol - 4 Issue - 4

Toxicology Research Vol - 4 Issue - 3

Toxicology Research Vol - 4 Issue - 2

Toxicology Research Vol - 4 Issue - 1

Toxicology Research Vol -3

Toxicology Research Vol -2

Toxicology Research Vol -1

Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Sharma, Love Tasduq, Sheikh A. Nissar, Ashraf U.
Copyright Year	2015
Abstract	The aim of the present investigation was to study the events associated with palmitic acid induced metabolic and lipotoxic changes in human hepatoma cells (Hep3B, Huh7 and HepG2). The cells were lipid-overloaded with varied concentrations of saturated fatty acid (palmitic acid). It was observed that (a) SFA induced significant metabolic changes including CD36/FAT, SREBP1, PPARγ and SCD1 upregulation and decreased Mttp levels in HepG2 and Huh7 cells but not in Hep3B cells; (b) the toxic manifestations including changes in Ca2+ levels, mitochondrial dysfunction and activation of the PERK-eIF2α-CHOP pathway due to SFA were more pronounced in Huh7 and Hep3B cells as compared to HepG2 cells; (c) induction of CYP2E1 and oxidative stress by SFA was observed in all three hepatoma cells. Interestingly, CYP2E1 overexpressing cells (E47 cells) were found to be significantly sensitized towards lipotoxicity; and (d) metabolic and toxic manifestations including altered blood biochemistry, ER stress and CYP2E1 induction were evident in C57BL/6J mice fed with a diet rich in cholesterol and saturated fatty acids (2% cholesterol/12% SFA-HC/HF) for 16 weeks (the model used to confirm the key events taking place in the in vitro system). Histopathological analysis of livers of HC/HF fed mice showed micro- and macro-vesicular steatosis, hepatocyte ballooning, infiltration of neutrophils and prominence of Kupffer cells. In conclusion our data suggest that palmitic acid alters lipid metabolism, causes oxidative stress and disturbs the intracellular Ca2+ balance that terminates in CYP2E1 induction, ER stress and lipoapoptosis.
Starting Page	1344
Ending Page	1358
Page Count	15
File Format	HTM / HTML PDF
ISSN	2045452X
Volume Number	4
Issue Number	5
Journal	Toxicology Research
DOI	10.1039/c5tx00101c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Peroxisome proliferator-activated receptor gamma Oxidative stress Biochemistry Metabolism Cholesterol Lipid SFA Steatosis Kupffer cell Hepatocyte Fatty acid Hydrofluoric acid Fat CD36 CYP2E1 Neutrophil The Cells Endoplasmic reticulum
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells

Role of CD36 in Palmitic Acid Lipotoxicity in Neuro-2a Neuroblastoma Cells

Palmitic acid induces intestinal lipid metabolism disorder, endoplasmic reticulum stress and inflammation by affecting phosphatidylethanolamine content in large yellow croaker Larimichthys crocea

Palmitic Acid Induces Endoplasmic Reticulum Stress In AML12 Liver Cells

The role of ER stress in lipid metabolism and lipotoxicity

Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis

Alteration of Endoplasmic Reticulum Lipid Rafts Contributes to Lipotoxicity in Pancreatic β-Cells

Vanadium(IV)-Chlorodipicolinate Protects against Hepatic Steatosis by Ameliorating Lipid Peroxidation, Endoplasmic Reticulum Stress, and Inflammation

Role of hepatocyte S6K1 in palmitic acid-induced endoplasmic reticulum stress, lipotoxicity, insulin resistance and in oleic acid-induced protection.

Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells

Similar Documents

Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells

Role of CD36 in Palmitic Acid Lipotoxicity in Neuro-2a Neuroblastoma Cells

Palmitic acid induces intestinal lipid metabolism disorder, endoplasmic reticulum stress and inflammation by affecting phosphatidylethanolamine content in large yellow croaker Larimichthys crocea

Palmitic Acid Induces Endoplasmic Reticulum Stress In AML12 Liver Cells

The role of ER stress in lipid metabolism and lipotoxicity

Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis

Alteration of Endoplasmic Reticulum Lipid Rafts Contributes to Lipotoxicity in Pancreatic β-Cells

Vanadium(IV)-Chlorodipicolinate Protects against Hepatic Steatosis by Ameliorating Lipid Peroxidation, Endoplasmic Reticulum Stress, and Inflammation

Role of hepatocyte S6K1 in palmitic acid-induced endoplasmic reticulum stress, lipotoxicity, insulin resistance and in oleic acid-induced protection.

Palmitic acid induced lipotoxicity is associated with altered lipid metabolism, enhanced CYP450 2E1 and intracellular calcium mediated ER stress in human hepatoma cells