NDLI: Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method

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Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method

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Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method

Content Provider	Royal Society of Chemistry (RSC)
Author	Miyamoto, Tomofumi Tanaka, Hiroyuki Yusakul, Gorawit Morimoto, Satoshi Sakamoto, Seiichi Putalun, Waraporn Usui, Kazuteru Tsuneura, Yumi
Copyright Year	2017
Abstract	Harringtonine (HT) is a promising natural product that is mainly isolated from plants of the genus Cephalotaxus. Due to its remarkable antileukemic activities, HT has been utilized clinically in China for the treatment of acute promyelocytic leukemia (APL). No antibody that recognizes free HT has been reported to date due to the difficulty of preparing antigen conjugates in which haptens bind to a carrier protein. To overcome this difficulty, we focused on sodium periodate (NaIO4), which catalyzes unique oxidative reactions; the resulting conjugates enabled the production of a highly specific monoclonal antibody (MAb) against HT (MAb 1D2) and the establishment of an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the determination of HT. Further analysis revealed that MAb 1D2 was produced by the HT3 (8-carbonyl HT)-based conjugate antigen; HT3 was synthesized by a NaIO4-mediated oxidative reaction. The minimum detectable concentration for HT in the icELISA system was found to be 0.76 ng mL−1, which is approximately 13 to 160 times more sensitive than a conventional HPLC system. Several validation analyses revealed that the icELISA using MAb 1D2 is sufficiently accurate, reliable, and sensitive to assess small amounts of HT in plant samples.
Starting Page	1140
Ending Page	1148
Page Count	9
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	7
Journal	The Analyst
DOI	10.1039/c6an02751b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Natural product Cephalotaxus Monoclonal antibody High-performance liquid chromatography Protein Antigen Acute promyelocytic leukemia China Genus Sodium periodate Catalysis Carbonyl group APL ELISA
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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