NDLI: Phase separation of siRNA–polycation complex and its effect on transfection efficiency

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Soft Matter Vol - 9 Issue - 6

Soft Matter Vol - 9 Issue - 5

Phase separation of siRNA–polycation complex and its effect on transfection efficiency

Content Provider	Royal Society of Chemistry (RSC)
Author	Liu, Jie Luo, Ying Liang, Dehai Xia, Yuqiong Shi, Tao Zhou, Jihan
Copyright Year	2013
Abstract	Polycations and cationic lipids have been widely employed as non-viral vectors for siRNA delivery. However, the stability of siRNA–polycation complexes is still rarely reported. Using highly charged dendrimer poly(amidoamine) (PAMAM) as an example, we studied the complexation of siRNA with a polycation by laser light scattering and atomic force microscopy. PAMAM forms a complex of more than 100 nm in diameter with siRNA right after mixing. However, the complex is not stable. It experiences an interesting kinetic process of aggregation, followed by phase separation. The rate of phase separation is related to the chemical composition of the polycations. Gene expressions on A549 cells and Hela cells demonstrate that the transfection efficiency decreases with the kinetics of the complexes. Cross-linking of the primary amino group of the PAMAM stabilizes the siRNA complex and causes the transfection results to be steady with time.
Starting Page	2262
Ending Page	2268
Page Count	7
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	9
Issue Number	7
Journal	Soft Matter
DOI	10.1039/c2sm27498a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Polyelectrolyte Dendrimer Transfection Gene Atomic-force microscopy Hela Province Scattering Laser Small interfering RNA Poly(amidoamine)
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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