NDLI: Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector

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Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector

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Soft Matter Vol - 8 Issue - 6

Soft Matter Vol - 8 Issue - 5

Self-assembled cationic triblock copolymer mPEG-b-PDLLA-b-PDMA nanoparticles as nonviral gene vector

Content Provider	Royal Society of Chemistry (RSC)
Author	Yue, Xinye Xing, Jinfeng Zhang, Biao Deng, Liandong Yang, Jun Zhang, Qiang Dong, Anjie Zhang, Wendi Guo, Shutao
Copyright Year	2012
Abstract	In this study, well-defined amphiphilic cationic triblock copolymers with different lengths of polycationic chain, methoxy poly(ethylene glycol)-b-poly(D,L-lactide)-b-poly(2-dimethylaminoethyl methacrylate) (mPEG-b-PDLLA-b-PDMA), were synthesized and evaluated as carriers for gene delivery. The prepared copolymers can self-assemble into spherical core–shell nanoparticles (NPs). The NPs have a very low critical micelle concentration (CMC) value with only 0.025 mg mL−1. Both copolymers can completely condense the pDNA into spherical complexes when the N/P ratio is equal to or above 3. Bovine serum albumin challenging and DNase I protection assay results demonstrate the mPEG-b-PDLLA-b-PDMA NPs can effectively protect the DNA against protein and nucleases. MTT assay results indicate that mPEG-b-PDLLA-b-PDMA NP/pDNA complexes exhibit obviously lower cytotoxicity compared with commercial gene transfection reagent Lipofectamine 2000/pDNA complexes. Subsequently, in vitro gene transfection studies in HeLa cells without serum show that mPEG113-b-PDLLA10-b-PDMA120 NP/pDNA complexes exhibit higher transfection efficiency than Lipofectamine 2000. Although the transfection efficiency is lower than that in the absence of serum, mPEG113-b-PDLLA10-b-PDMA120 NPs still display equivalent gene transfection efficiency compared to Lipofectamine 2000 when N/P ratio is above 15 in DMEM with 10% serum.
Starting Page	2252
Ending Page	2260
Page Count	9
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	8
Issue Number	7
Journal	Soft Matter
DOI	10.1039/c2sm07068e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Ethylene glycol Bovine serum albumin Cytotoxicity MTT MTT assay Reagent Protein HeLa Critical micelle concentration Deoxyribonuclease I Transfection Gene DNA CMC Amphiphile Methoxy group
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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