NDLI: AFM measurements and lipid rearrangements: evidence from red blood cell shape changes

Please wait, while we are loading the content...

Miniemulsion template polymerization to prepare a sub-micrometer porous polymeric monolith with an inter-connected structure and very high mechanical strength

Translational and rotational diffusion of micrometer-sized solid domains in lipid membranes

Interaction of sedimenting drops in a miscible solution – formation of heterogeneous toroidal-spiral particles

Wet versus dry adhesion of biomimetic mushroom-shaped microstructures

Effect of ions on confined near-critical binary aqueous mixture

Mixed protein–polysaccharide interfacial layers: effect of polysaccharide charge distribution

Printable ultrathin substrates formed on a concave–convex underlayer for highly flexible membrane-type electrode stickers

Non-linear alignment dynamics in suspensions of platelets under rotating magnetic fields

Directional interactions in semiflexible single-chain polymer folding

Concurrent supramolecular gelation and fluorescence turn-on triggered by coordination of silver ion

A crystal engineering rationale in designing a Cd^II coordination polymer based metallogel derived from a C3 symmetric tris-amide-tris-carboxylate ligand

Microcontact printing of polyelectrolytes on PEG using an unmodified PDMS stamp for micropatterning nanoparticles, DNA, proteins and cells

Injectable in situ-forming hydrogels for a suppression of drug burst from drug-loaded microcapsules

Synthesis and pH- and salinity-controlled self-assembly of novel amphiphilic block-gradient copolymers of styrene and acrylic acid

Soluble surfactant spreading on spatially confined thin liquid films

A comparison of different experimental methods for investigating the mechanical properties of plane polysiloxane membranes and capsule walls

Probing the dynamics of particles in an aging dispersion using diffusing wave spectroscopy

Configuration of nonspherical amphiphilic particles at a fluid–fluid interface

Steady shear microstructure in dilute colloid–polymer mixtures

Mesh size distribution determination of interpenetrating polymer network hydrogels

AFM measurements and lipid rearrangements: evidence from red blood cell shape changes

Back cover

Soft Matter Vol - 8 Issue - 19

Soft Matter Vol - 8 Issue - 9

Soft Matter Vol - 8 Issue - 8

Soft Matter Vol - 8 Issue - 7

Soft Matter Vol - 8 Issue - 6

Soft Matter Vol - 8 Issue - 5

AFM measurements and lipid rearrangements: evidence from red blood cell shape changes

Content Provider	Royal Society of Chemistry (RSC)
Author	Hernández-Machado, Aurora Lázaro, Guillermo R. Espada, José Manuel Cárdenas Díaz de Pagonabarraga, Ignacio Melzak, Kathryn A. Toca-Herrera, José L.
Copyright Year	2012
Abstract	Application of force to echinocytes during atomic force microscopy measurements was shown to be able to convert the cells to stable discocyte shapes. The echinocyte shape is associated with a relative excess of the area of the outer leaflet of the cell membrane; the AFM measurements are therefore associated with a change in the relative areas of the inner and outer membrane leaflets. It was hypothesized that localised damage in the lipid bilayer that is caused by an AFM tip can permit the lipids to flip-flop between the two membrane leaflets, thus changing their relative areas. The conditions in which AFM measurements on cells could induce shape changes were investigated both experimentally and by modelling. The relative area change of the membrane leaflets, attributed here to lipid movement, was characterised in terms of the membrane energy levels; membrane energy was calculated using a version of the area-difference-elasticity model that was applied to predetermined shapes, rather than being used to generate shapes as solutions found at the energy minima. Shapes were generated by rotation of Cassini ovals with a superimposed undulation in order to generate spikes similar to those of the echinocytes. The membrane energy was considered as a function of the membrane curvature, the area difference between the two membrane leaflets, and the deformation of the cytoskeleton. This led to the conclusions that the minimisation of the membrane energy causes the lipid translocation, with the relaxation of the cytoskeleton being a significant driving force.
Starting Page	7716
Ending Page	7726
Page Count	11
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	8
Issue Number	29
Journal	Soft Matter
DOI	10.1039/c2sm25530h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cassini Atomic-force microscopy Cell membrane French cartography Lipid bilayer Cytoskeleton Echinocyte Curvature Lipid Flip-flops
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Investigation of Red Blood Cells by Atomic Force Microscopy

Stomatocyte-discocyte-echinocyte sequence of the human red blood cell: evidence for the bilayer- couple hypothesis from membrane mechanics.

Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy.

Diabetology original investigation changes in red blood cell.

Quantitative assessment of lipid bilayer-cytoskeleton coupling in red blood cell membrane

Stomatocyte-discocyte-echinocyte sequence of the human red blood cell: evidence for the bilayer- couple hypothesis from membrane mechanics.

Membrane fluidization & eryptotic properties of hesperidin–copper complex

On the mechanism of stomatocyte–echinocyte transformations of red blood cells: experiment and theoretical model (2003).

Stomatocyte–discocyte–echinocyte sequence of the human red blood cell: Evidence for the bilayer– couple hypothesis from membrane mechanics

AFM measurements and lipid rearrangements: evidence from red blood cell shape changes

Similar Documents

Investigation of Red Blood Cells by Atomic Force Microscopy

Stomatocyte-discocyte-echinocyte sequence of the human red blood cell: evidence for the bilayer- couple hypothesis from membrane mechanics.

Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy.

Diabetology original investigation changes in red blood cell.

Quantitative assessment of lipid bilayer-cytoskeleton coupling in red blood cell membrane

Stomatocyte-discocyte-echinocyte sequence of the human red blood cell: evidence for the bilayer- couple hypothesis from membrane mechanics.

Membrane fluidization & eryptotic properties of hesperidin–copper complex

On the mechanism of stomatocyte–echinocyte transformations of red blood cells: experiment and theoretical model (2003).

Stomatocyte–discocyte–echinocyte sequence of the human red blood cell: Evidence for the bilayer– couple hypothesis from membrane mechanics

AFM measurements and lipid rearrangements: evidence from red blood cell shape changes