NDLI: In-plane homogeneity and lipid dynamics in tethered bilayer lipid membranes (tBLMs)

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Soft Matter Vol - 6 Issue - 5

Soft Matter Vol - 6 Issue - 4

Soft Matter Vol - 6 Issue - 3

In-plane homogeneity and lipid dynamics in tethered bilayer lipid membranes (tBLMs)

Content Provider	Royal Society of Chemistry (RSC)
Author	Shenoy, Siddharth Deserno, Markus Vanderah, David J. Fitzpatrick, James Moldovan, Radu Lösche, Mathias
Copyright Year	2010
Abstract	Tethered bilayer lipid membranes (tBLMs) were prepared by the self-assembly of thiolated lipidic anchor molecules on gold, followed by phospholipid precipitation via rapid solvent exchange. They were characterized by their in-plane structure, dynamics and dielectric properties. We find that the in-plane homogeneity and resistivity of the tBLMs depend critically on a well-controlled sample environment during the rapid solvent-exchange procedure. The in-plane dynamics of the systems, assessed by fluorescence correlation spectroscopy (FCS) as the diffusivity of free, labeled phospholipid dissolved in the membrane, depend on the density of the lipidic anchors in the bilayer leaflet proximal to the substrate as well as on details of the molecular structure of the anchor lipid. In DOPC tBLMs in which tethers are laterally dilute (sparsely tethered bilayer lipid membranes, stBLMs), measured diffusivities, D ≈ 4 µm2 s−1, are only slightly greater than those reported in physisorbed bilayers (M. Przybylo, J. Sýkora, J. Humpolíckova, A. Benda, A. Zan and M. Hof, Langmuir, 2006, 22, 9096–9099). However, when we distinguish label diffusion in the proximal and in the distal bilayer leaflets, we observe distinct diffusivities, D ≈ 2 µm2 s−1 and 7 µm2 s−1, respectively. The value observed in the distal leaflet is identical to that in free membranes. stBLMs completed with phytanoyl lipids (DPhyPC) show consistently lower label diffusivity than those completed with unsaturated chains (DOPC). As the length of the tether chain increases, a reduction in the apparent diffusivity is observed, which we interpret as an increased propensity of the proximal bilayer leaflet to host free lipid. We also investigated preparation conditions that control whether the tBLMs are laterally homogeneous, as assessed by optical microscopy. In laterally heterogeneous bilayers, the label diffusivity varies only by a factor of ∼2 to 4, indicating that the regions in the bilayers with different label solubilities do not correspond to distinct phases, such as a fluid phase coexisting with a gel phase.
Starting Page	1263
Ending Page	1274
Page Count	12
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	6
Issue Number	6
Journal	Soft Matter
DOI	10.1039/b919988h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	kora Zan Optical microscope Phospholipid Dielectric Lipid Przybylo Electrical resistivity and conductivity Self-assembly Irving Langmuir Langmuir FCS ckova Franz Benda Benda Diffusion Solvent Hof Fluorescence correlation spectroscopy
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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