NDLI: Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

Please wait, while we are loading the content...

Controllable self-assemblies of micro/nano-tubes and vesicles from arylamides and their applications as templates to fabricate Pt micro/nano-tubes and hollow Pt nanospheres

Static and dynamic control of phase separation structures in nanoparticles of polymer blends

Plateau-Rayleigh instability in a torus: formation and breakup of a polymer ring

In-plane homogeneity and lipid dynamics in tethered bilayer lipid membranes (tBLMs)

Fluctuations and elastic properties of lipid membranes in the gel L_β′ state: a coarse-grained Monte Carlo study

Phase behavior of polyisoprene-poly(butylene oxide) and poly(ethylene-alt-propylene)-poly(butylene oxide) block copolymers

Microemulsion-based organogels containing inorganic nanoparticles

Atomistic simulations of the wetting behavior of nanodroplets of water on homogeneous and phase separated self-assembled monolayers

Effects of valences of salt ions at various concentrations on charged dendrimers

Formation of square lamellae by self-assembly of long-chain bolaphospholipids in water

Self-assembly of Nafion®/poly(vinyl alcohol) at pH = 1.2 and Nafion®/poly(allyl amine) at pH = 11

Influence of polymer network-metal ion complexation on the swelling behaviour of new gels with incorporated α-amino acid groups

Adhesion of yield stress fluids

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

Back cover

Soft Matter Vol - 6 Issue - 5

Soft Matter Vol - 6 Issue - 4

Soft Matter Vol - 6 Issue - 3

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

Content Provider	Royal Society of Chemistry (RSC)
Author	Vreeland, Wyatt N. Zook, Justin M.
Copyright Year	2010
Abstract	Microfluidic hydrodynamic focusing of an alcohol–lipid mixture into a narrow fluid stream by two oblique buffer streams provides a controlled and reproducible method of forming phospholipid bilayer vesicles (i.e., liposomes) with relatively monodisperse and specific size ranges. Previous work has established that liposome size can be controlled by changing the relative and absolute flow rates of the fluids. In other previous work, a kinetic (non-equilibrium) theoretical description of the detergent dilution liposome formation method was developed, in which planar lipid bilayer discs aggregate until they become sufficiently large to close into spherical liposomes. In this work, we show that an approximation of the kinetic theory can help explain liposome formation for our microfluidic method. This approximation predicts that the liposome radius should be approximately proportional to the ratio of the membrane bending elasticity modulus to the line tension of the hydrophobic edges of the lipid bilayer disc. In combination with very fast microfluidic mixing, this theory enables a new method to measure the ratio of the elasticity modulus to the line tension of membranes. The theory predicts that the temperature should change the liposome size primarily as a result of its effect on the ratio of the membrane bending elasticity modulus to the line tension, in contrast to previous work on microdroplet and microbubble formation, which showed that the effect of temperature on droplet/bubble size was primarily due to viscosity changes. In agreement with theory, most membrane compositions form larger liposomes close to or below the gel-to-liquid crystalline phase transition temperature, where the membrane elasticity modulus is much larger, and they have a much smaller dependence of size on temperature far above the transition temperature, where the membrane elasticity modulus is relatively constant. Other parameters modulated by the temperature (e.g., viscosity, free energy, and diffusion coefficients) appear to have little or no effect on liposome size, because they have counteracting effects on the lipid aggregation rate and the liposome closure time. Experiments are performed using phospholipids with varying hydrophobic acyl chain lengths that have phase transition temperatures ranging from −1 °C to 55 °C, so that the temperature dependence is examined below, above, and around the transition temperature. In addition, the effect of IPA stabilizing the edges of the bilayer discs can be examined by comparing the liposome sizes obtained at different flow-rate ratios. Finally, polydispersity is shown to increase as the median liposome size increases, regardless of whether the change in size is due to changing temperature or flow-rate ratio.
Starting Page	1352
Ending Page	1360
Page Count	9
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	6
Issue Number	6
Journal	Soft Matter
DOI	10.1039/b923299k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Dispersity Viscosity Detergent Kinetic theory of gases Liposome Hydrophobe Lipid Thermodynamic free energy Isopropyl alcohol Soap bubble Lipid bilayer Correlation and dependence Diffusion Phase transition
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

Analysis of the Diffusion Process by pH Indicator in Microfluidic Chips for Liposome Production

Hydrodynamic Focusing Effect on Two- (2008)

Hydrodynamic focusing effect on two- (2008).

Effects of phase transition of a lipid bilayer on dynamics of bubble liposomes

Emulsification in a microfluidic flow-focusing device: effect of the viscosities of the liquids.

Nonelectrolyte diffusion across lipid bilayer systems.

Liposome Nanoparticle Generation with Flow Focusing Microfluidic Device

Two-dimensional hydrodynamic focusing in a simple microfluidic device

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

Similar Documents

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device

Analysis of the Diffusion Process by pH Indicator in Microfluidic Chips for Liposome Production

Hydrodynamic Focusing Effect on Two- (2008)

Hydrodynamic focusing effect on two- (2008).

Effects of phase transition of a lipid bilayer on dynamics of bubble liposomes

Emulsification in a microfluidic flow-focusing device: effect of the viscosities of the liquids.

Nonelectrolyte diffusion across lipid bilayer systems.

Liposome Nanoparticle Generation with Flow Focusing Microfluidic Device

Two-dimensional hydrodynamic focusing in a simple microfluidic device

Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodynamic focusing device