NDLI: Interactions of cucurbit[6,7]urils with human serum albumin and their effects on zaltoprofen transportation

Content Provider	Royal Society of Chemistry (RSC)
Author	Yang, Hongqin Huang, Yanmei He, Jiawei Li, Hui Xu, Kailin Wang, Qing Li, Shanshan
Copyright Year	2016
Abstract	Cucurbit[6,7]urils (CB[6,7]s) have been extensively investigated because of their characteristic inclusion ability. To enhance our understanding of the behaviors of CB[6,7]s in biological environments and explore their effects on drug transportation, multi-spectroscopic and molecular docking methods were applied to examine the binding mechanism of CB[6,7]s with human serum albumin (HSA) and their interference on the HSA–zaltoprofen (ZPF) binding system. 1H NMR spectra revealed the complexes that formed between CB[6,7]s and HSA, as well as between ZPF and HSA. The fluorescence of HSA was quenched by ZPF through a combined static and dynamic mechanism. The added CB[6,7]s did not significantly affect the energy transfer efficiency, but decreased the HSA–ZPF binding constant. The binding stoichiometry of ZPF with HSA was 1 : 1, and this value remained unchanged when CB[6,7]s were added. Circular dichroism spectroscopy, synchronous and 3D fluorescence spectroscopy demonstrated that CB[6,7]s slightly influenced the native secondary structure of HSA and its ZPF-induced unfolding. Further molecular docking suggested that CB[6,7]s preferred to bind to the HSA surface, and ZPF was primarily located in an L-shaped cavity in subdomain IB. Therefore, CB[6,7]s elicited minor damage to the structure of HSA and weak interferance with drug transportation.
Starting Page	85811
Ending Page	85819
Page Count	9
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	6
Issue Number	89
Journal	RSC Advances
DOI	10.1039/c6ra17508b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Fluorescence spectroscopy Human serum albumin Stoichiometry Spectroscopy Citizens band radio Docking (molecular) Binding constant Biomolecular structure Circular dichroism Three-dimensional space HSA Nuclear magnetic resonance
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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