NDLI: Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique

Content Provider	Royal Society of Chemistry (RSC)
Author	Pires, Pedro Shi, Xiangyang Rodrigues, João Xiao, Shili Ramalingam, Kirthiga Tomás, Helena Castro, Rita
Copyright Year	2016
Abstract	A gene delivery platform for potential use in tissue engineering applications was developed by surface functionalization of biodegradable electrospun poly(lactic-co-glycolic acid) (PLGA) fibers with nanolayers of chitosan (cationic polymer) and alginate (anionic polymer) using the Layer-by-Layer (LbL) technique. The developed system not only supported the attachment and growth of human Mesenchymal Stem Cells (hMSCs), but also was capable of delivering pDNA/dendrimer complexes and inducing cell differentiation towards the osteogenic lineage when a pDNA codifying for human Bone Morphogenetic Protein-2 (BMP-2) was used. Beyond providing a means for pDNA/dendrimer complex immobilization, the polyelectrolyte coating conferred sustained release properties to the scaffold that resulted in pDNA protection from degradation. The polyelectrolyte coating, by itself, also contributed to enhance cell differentiation.
Starting Page	97116
Ending Page	97128
Page Count	13
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	6
Issue Number	99
Journal	RSC Advances
DOI	10.1039/c6ra22444j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biodegradation Polyelectrolyte Dendrimer Gene Bone morphogenetic protein 2 Tissue engineering Carboxylic acid Alginic acid Polymer Chitosan
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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