NDLI: Environmentally responsive amino acid-bioconjugated dynamic covalent copolymer as a versatile scaffold for conjugation

Content Provider	Royal Society of Chemistry (RSC)
Author	Wu, Libin Liu, Lingzhi Liu, Li Yang, Shixia Wang, Lin Wang, Xiaobei Zhao, Hanying
Copyright Year	2015
Abstract	Biodynamers display the properties of biomolecules with the adaptive nature of dynamic covalent polymers and have great potential as “smart” materials. The bioconjugation of L-tyrosine hydrazide to the end of bisaldehyde-functionalized thermoresponsive copolymer chains via reversible acylhydrazone linkage generated a biodynamer with thermo/pH-responsive and adaptive features. Because of the introduction of amino acid end groups, the biodynamer had an isoelectric point (IEP) at pH 4.70 and possessed a pH-dependent LCST. The properties of the biodynamer were tunable through chain exchange reactions with other hydrazine or hydrazide molecules. The biodynamer endowed biological recognition by exchange with biotin hydrazide. The complex formed by the biotinylated dynamer and streptavidin demonstrated pH-responsive features. Because of the versatile reactivity of the phenolic moiety, this biodynamer provided a reactive scaffold for further modification. The cyclic diazodicarboxamide was attached to the biodynamer through the tyrosine-click reaction. Protein bioconjugate with pH-responsive and adaptive features was prepared via HRP-catalyzed coupling reaction.
Starting Page	30456
Ending Page	30463
Page Count	8
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	5
Issue Number	39
Journal	RSC Advances
DOI	10.1039/c5ra00192g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Tyrosine Carboxylic acid Isoelectric point Hydrazide Streptavidin Covalent bond Bioconjugation Biotin Copolymer Hydrazine Coupling reaction
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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