NDLI: A fluorescence assay for the trace detection of protamine and heparin

Content Provider	Royal Society of Chemistry (RSC)
Author	Su, Xingguang Pang, Shu Liu, Siyu
Copyright Year	2014
Abstract	Here we designed a fluorescence sensing strategy for the determination of protamine, heparin and trypsin. A fluorescein isothiocyanate (FITC)-labeled DNA probe could wrap around protamine molecules tightly via electrostatic interactions accompanied by the fluorescence quenching of the DNA probe. In the presence of heparin, protamine preferred to bind to heparin instead of DNA due to the stronger affinity of heparin to protamine, and the fluorescence could be restored. Based on the fact that the protamine which is rich in arginine could be hydrolyzed by the addition of trypsin, the fluorescence intensity of the DNA/protamine complex could also be linked to the trypsin concentration. By measuring the fluorescence intensity changes, the concentrations of protamine, heparin and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 2.5 to 17.5 ng mL−1, 0.156–1.875 ng mL−1 and 62.5 to 10 000 ng mL−1 with the low detection limit of 2.2 ng mL−1, 0.078 ng mL−1 and 30.2 ng mL−1 for protamine, heparin and trypsin, respectively.
Starting Page	25857
Ending Page	25862
Page Count	6
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	4
Issue Number	49
Journal	RSC Advances
DOI	10.1039/c4ra02936d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Fluorescein isothiocyanate Trypsin Arginine Protamine Hybridization probe DNA Fluorescence Isothiocyanate Heparin
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A fluorescence assay for the trace detection of protamine and heparin

A DNA-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases

A simple fluorescence assay for trypsin through a protamine-induced carbon quantum dot-quenching aggregation platform.

A novel exonuclease III-aided amplification assay based on a graphene platform for sensitive detection of adenosine triphosphate

Determination of trace deoxyribonucleic acid by using fluorescein isothiocyanate-phenosafranine (2010).

Bovine serum albumin-capped CdS quantum dots as an inner-filter effect sensor for rapid detection and quantification of protamine and heparin

Guanine-based chemiluminescence resonance energy transfer biosensing platform for the specific assay of uracil-DNA glycosylase activity

Detection of immunoglobulin G based on nanoparticle surface energy transfers from fluorescein isothiocyanate to gold nanoparticles

Cell-free bioassay for measurement of dioxins based on fluorescence enhancement of fluorescein isothiocyanate-labeled DNA probe.

A fluorescence assay for the trace detection of protamine and heparin

Similar Documents

A fluorescence assay for the trace detection of protamine and heparin

A DNA-directed covalent conjugation fluorescence probe for in vitro detection of functional matrix metalloproteinases

A simple fluorescence assay for trypsin through a protamine-induced carbon quantum dot-quenching aggregation platform.

A novel exonuclease III-aided amplification assay based on a graphene platform for sensitive detection of adenosine triphosphate

Determination of trace deoxyribonucleic acid by using fluorescein isothiocyanate-phenosafranine (2010).

Bovine serum albumin-capped CdS quantum dots as an inner-filter effect sensor for rapid detection and quantification of protamine and heparin

Guanine-based chemiluminescence resonance energy transfer biosensing platform for the specific assay of uracil-DNA glycosylase activity

Detection of immunoglobulin G based on nanoparticle surface energy transfers from fluorescein isothiocyanate to gold nanoparticles

Cell-free bioassay for measurement of dioxins based on fluorescence enhancement of fluorescein isothiocyanate-labeled DNA probe.

A fluorescence assay for the trace detection of protamine and heparin