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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Wang, Mengfan Zhang, Mingjia Yang, Jifeng Su, Rongxin He, Zhimin Du, Ruoyu Qi, Wei Huang, Renliang |
| Copyright Year | 2013 |
| Abstract | The measurement of molecular parameters, such as molecular weight and degrees of polymerisation, in lignocellulosic feedstock are critical during the hydrolysis process, because following the changes in these parameters can provide insight into the mechanism of hydrolysis. This work employs SEC-MALLS to monitor the changes in the absolute values of these parameters of lignocellulosic samples during enzymatic hydrolysis. The lignocellulosic samples were directly dissolved in 8.0% LiCl/DMAc without any change. The number- and weight-average molecular weights of the cellulose fraction decreased initially and increased slightly thereafter, indicating that a “layer-by-layer” mechanism is adapted for enzymatic hydrolysis of the cellulose fraction of lignocellulose. Similar changes in the molecular weights of the hemicellulose fraction indicates that hemicellulose and cellulose are always together in the recalcitrant substrates during enzymatic hydrolysis. Finally, the reaction mechanism of cellulase on lignocellulose was defined by SEC-MALLS analysis combining HPLC and XRD data. |
| Starting Page | 1871 |
| Ending Page | 1877 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 20462069 |
| Volume Number | 3 |
| Issue Number | 6 |
| Journal | RSC Advances |
| DOI | 10.1039/c2ra21781c |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Hydrolysis X-ray crystallography Hemicellulose Lithium chloride Cellulase Cellulose Reaction mechanism High-performance liquid chromatography Lignocellulosic biomass HPLC |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Chemical Engineering |
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