NDLI: The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156

Please wait, while we are loading the content...

Probing photochromic properties by correlation of UV-visible and infra-red absorption spectroscopy: a case study with cis-1,2-dicyano-1,2-bis(2,4,5-trimethyl-3-thienyl)ethene

The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156

Quantitative investigations of cation complexation of photochromic 8-benzothiazole-substituted benzopyran: towards metal-ion sensors

Photo-controlled phase separation and mixing of a mixture of water and 2-butoxyethanol caused by photochromic isomerisation of spiropyran

Spiropyrans as molecular optical switches

Photoinduced shape changes of diarylethene single crystals: correlation between shape changes and molecular packing

Functional interaction structures of the photochromic retinal protein rhodopsin

Facile synthesis and characterization of new photochromic trans-dithienylethenes functionalized with pyridines and fluorenes

Higher resolution in localization microscopy by slower switching of a photochromic protein

Optical control of quantum dot luminescence via photoisomerization of a surface-coordinated, cationic dithienylethene

Low-temperature switching by photoinduced protonation in photochromic fluorescent proteins

Back cover

Photochemical & Photobiological Sciences Vol -8

Photochemical & Photobiological Sciences Vol -7

Photochemical & Photobiological Sciences Vol -6

Photochemical & Photobiological Sciences Vol -5

Photochemical & Photobiological Sciences Vol -4

Photochemical & Photobiological Sciences Vol -3

Photochemical & Photobiological Sciences Vol -2

The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156

Content Provider	Royal Society of Chemistry (RSC)
Author	Heberle, Joachim Radu, Ionela Bamann, Christian Gossing, Michael Mollard, Gabriele Fischer Bamberg, Ernst Nack, Melanie
Copyright Year	2010
Abstract	The light-gated cation channel Channelrhodopsin-2 (ChR2), a retinylidene protein found in the eye-spot of Chlamydomonas reinhardtii, became an optogenetic tool to trigger neurophysiological responses by light and, thus, revolutionized spatio-temporal studies of such processes. The reaction mechanism still remains elusive but recent vibrational spectroscopic experiments started to resolve details of the associated structural changes during the photocycle. Large alterations in the polypeptide backbone were observed by FT-IR spectroscopy that precede and succeed the opening and closing of the channel, respectively. However, the molecular switch that controls gating is still unknown. Here, we present difference spectra of the D156E mutant of ChR2 and assign the observed vibrational bands to crucial hydrogen bonding changes of this residue in various intermediate states of the photoreaction. By comparison with spectra of wild-type ChR2 and the C128T mutant and correlation to electrophysiological studies, we propose the DC gate as a crucial hydrogen-bonding interaction between D156 and C128 which may represent the valve of the channel.
Starting Page	194
Ending Page	198
Page Count	5
File Format	HTM / HTML PDF
ISSN	1474905X
Volume Number	9
Issue Number	2
Journal	Photochemical & Photobiological Sciences
DOI	10.1039/b9pp00157c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Retinylidene protein Molecular switch Chlamydomonas reinhardtii Channelrhodopsin Mutant Reaction mechanism Ion Fourier-transform infrared spectroscopy Peptide Hydrogen bond
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Monitoring light-induced structural changes of Channelrhodopsin-2 by UV-visible and Fourier transform infrared spectroscopy.

Vibrational dynamics of the hydrogen bond in H(2)S-HF: Fourier-transform-infrared spectra and ab initio theory.

Theoretical And Spectroscopic Studies On Weakly Bound Complexes And Acetylene

Mutation induced modulation of hydrogen bonding to p700 studied using ftir difference spectroscopy† (2003).

Molecular Recognition via Hydrogen Bonding in Supramolecular Complexes: A Fourier Transform Infrared Spectroscopy Study

Interaction between Soluble and Membrane-Embedded Potassium Channel Peptides Monitored by Fourier Transform Infrared Spectroscopy (2012)

Chimeras of Channelrhodopsin-1 and -2 from Chlamydomonas reinhardtii Exhibit Distinctive Light-induced Structural Changes from Channelrhodopsin-2

Enhanced Lipid Production in Chlamydomonas reinhardtii Caused by Severe Iron Deficiency

Rapid Determination of Lipid Accumulation Under Sulfur Starvation in Chlamydomonas reinhardtii Microalga Using Fourier Transform Infrared ( FTIR ) spectroscopy

The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156

Similar Documents

Monitoring light-induced structural changes of Channelrhodopsin-2 by UV-visible and Fourier transform infrared spectroscopy.

Vibrational dynamics of the hydrogen bond in H(2)S-HF: Fourier-transform-infrared spectra and ab initio theory.

Theoretical And Spectroscopic Studies On Weakly Bound Complexes And Acetylene

Mutation induced modulation of hydrogen bonding to p700 studied using ftir difference spectroscopy† (2003).

Molecular Recognition via Hydrogen Bonding in Supramolecular Complexes: A Fourier Transform Infrared Spectroscopy Study

Interaction between Soluble and Membrane-Embedded Potassium Channel Peptides Monitored by Fourier Transform Infrared Spectroscopy (2012)

Chimeras of Channelrhodopsin-1 and -2 from Chlamydomonas reinhardtii Exhibit Distinctive Light-induced Structural Changes from Channelrhodopsin-2

Enhanced Lipid Production in Chlamydomonas reinhardtii Caused by Severe Iron Deficiency

Rapid Determination of Lipid Accumulation Under Sulfur Starvation in Chlamydomonas reinhardtii Microalga Using Fourier Transform Infrared ( FTIR ) spectroscopy

The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156