NDLI: Polymeric vesicles mimicking glycocalyx (PV-Gx) for studying carbohydrate

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhao, Yu Su, Lu Jiang, Ming Chen, Guosong
Copyright Year	2012
Abstract	Glycocalyx, the carbohydrate coat on cell surfaces, has been proved to be particularly important in a variety of biological events. In this work, polymeric vesicle mimicking of glycocalyx (PV-Gx), as a simplified model system, is achieved via our NCCM (non-covalently connected micelles) strategy. Briefly, a thermal responsive poly(N-isopropylacrylamide) with phenylboronic acid (BA) end (BA-PNIPAM) and two novel hydrophilic glycopolymers, PGal and PGlc are prepared by RAFT polymerization, where the latter are prepared from N-linked β-pyranoside monomers containing respective units of galactoside (Gal) and glucoside (Glc). Upon heating PNIPAM-BA and PGal (PGlc) self-assemble into vesicles V-PGal (V-PGlc), driven by the dynamic covalent bond between sugars and BA. Both V-PGal and V-PGlc could serve as our target artificial glycocalyx (PV-Gx) because they have sugar-coated surfaces and disperse stably in water. Dynamic light scattering (DLS) has been employed to monitor the binding process between the sugars on PV-Gx and three lectins i.e. Arachis hypogaea (PNA), Erythrina cristagalli (ECA) and Concanavalin A (Con A), concluding that the PV-Gx shows clear specificities in the sugar-protein interactions: V-PGal interacts well with PNA and ECA to form aggregates but not with ConA, while V-PGlc interacts with none of the lectins. The results clearly prove that the PV-Gx constructed from the polymers with well-defined sugar units is a new and promising platform for the study of carbohydrate-protein interactions in solution.
Starting Page	1560
Ending Page	1566
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599954
Volume Number	3
Issue Number	6
Journal	Polymer Chemistry
DOI	10.1039/c2py20110k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Erythrina PNA ECA Glucoside Polymerization Concanavalin A Glycocalyx Poly(N-isopropylacrylamide) Dynamic light scattering Covalent bond Galactoside Carbohydrate Hydrophile Arachis
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Bioengineering Biomedical Engineering Polymers and Plastics

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