NDLI: An enzyme-induced Au@Ag core–shell nanoStructure used for an ultrasensitive surface-enhanced Raman scattering immunoassay of cancer biomarkers

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An enzyme-induced Au@Ag core–shell nanoStructure used for an ultrasensitive surface-enhanced Raman scattering immunoassay of cancer biomarkers

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Nanoscale Vol - 9 Issue - 6

Nanoscale Vol - 9 Issue - 5

Nanoscale Vol - 9 Issue - 4

Nanoscale Vol - 9 Issue - 3

Nanoscale Vol - 9 Issue - 2

An enzyme-induced Au@Ag core–shell nanoStructure used for an ultrasensitive surface-enhanced Raman scattering immunoassay of cancer biomarkers

Content Provider	Royal Society of Chemistry (RSC)
Author	Huang, Cheng Zhi Zhen, Shu Jun Yang, Lin Gong, Min Gao, Ming Xuan Zhan, Lei
Copyright Year	2017
Abstract	Early detection of cancer is helpful for the control and prevention of diseases. Due to the low content of cancer biomarkers in the early disease phases, however, an ultrasensitive and selective method is critical. In this contribution, an ultrasensitive surface-enhanced Raman scattering (SERS) immunoassay is newly developed with the principle of introducing a common enzyme-induced deposition (EID) reaction to coat a silver layer on the surface of gold nanoparticles and to form a core–shell nanostructure of Au@Ag. By using alkaline phosphatase (ALP) to dephosphorylate its substrate, 2-phospho-L-ascorbic acid trisodium salt (AAP), to form vitamin C, silver ions could be reduced into silver atoms and coated on the surface of the AuNPs; a greatly enhanced SERS signal was then obtained. As a proof of concept, α-fetoprotein (AFP) was detected as a target, which is a biomarker of liver cancer. Excellent analytical performance of the SERS immunoassay could be achieved in the range from 0.5 to 100 pg mL−1 with a limit of detection of 0.081 pg mL−1 (3σ). Identical results could be obtained by using the newly proposed SERS immunoassay for the clinical detection of AFP in serum samples of patients to those clinically obtained by chemiluminescence immunoassays, demonstrating the potential applications of the developed method in clinical diagnosis.
Starting Page	2640
Ending Page	2645
Page Count	6
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	9
Issue Number	7
Journal	Nanoscale
DOI	10.1039/c6nr07979b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Immunoassay Alkaline phosphatase Biomarker Surface-enhanced Raman spectroscopy Hepatocellular carcinoma Nanostructure Chemiluminescence AFP Alpha-fetoprotein SERS Vitamin C Cancer
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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