NDLI: A colorimetric sensing platform based upon recognizing hybridization chain reaction products with oligonucleotide modified gold nanoparticles through triplex formation

Content Provider	Royal Society of Chemistry (RSC)
Author	Zou, Li Li, Ruimin Ling, Liansheng Zhou, Nian Luo, Yanwei Zhang, Manjun Wang, Jing
Copyright Year	2017
Abstract	A novel colorimetric sensing strategy for biomolecule assay has been developed, which integrates the signal amplification of the hybridization chain reaction (HCR) with the assembly of gold nanoparticles (AuNPs) through triplex formation. In the presence of targets, the HCR process can be triggered, the HCR products are specifically recognized by triplex-forming oligonucleotide (TFO) functionalized AuNPs, accompanying the aggregation of AuNPs and a dramatic absorbance change at 522 nm. In addition, the DNA hairpin probes can form rigid triplex structures with TFO-functionalized AuNPs in the absence of targets, resulting in a negligible background signal. By taking advantage of this new biosensor platform, a broad range of targets, involving nucleic acids, small molecules and proteins, have been successfully determined with high sensitivity and selectivity, which may hold great potential for practical application.
Starting Page	1986
Ending Page	1992
Page Count	7
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	9
Issue Number	5
Journal	Nanoscale
DOI	10.1039/c6nr09089c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biomolecule Chain reaction HCR DNA Biosensor Oligonucleotide TFO
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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A colorimetric sensing platform based upon recognizing hybridization chain reaction products with oligonucleotide modified gold nanoparticles through triplex formation