NDLI: Preparation and application of silver nanoparticle-functionalized magnetic graphene oxide nanocomposites

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Inside back cover

Back cover

Nanoscale Vol - 9 Issue - 3

Nanoscale Vol - 9 Issue - 2

Preparation and application of silver nanoparticle-functionalized magnetic graphene oxide nanocomposites

Content Provider	Royal Society of Chemistry (RSC)
Author	Wu, Qiong Jiang, Bo Zhang, Yukui Zhang, Lihua
Copyright Year	2017
Abstract	Although selective enrichment of glycopeptides from complex biological samples is indispensable for mass spectrometry (MS)-based glycoproteomics, it still remains a great challenge due to the low abundance of glycoproteins and suppression of non-glycopeptides. In this study, silver nanoparticle-functionalized magnetic graphene oxide nanocomposites (GO/Fe3O4/PEI/Ag) were synthesized. Silver nanoparticles were generated in situ on the surface of magnetic graphene oxide using polyethylenimine as a reducing and stabilizing agent. The resulting material was used as an adsorbent for selective enrichment of glycopeptides. GO/Fe3O4/PEI/Ag nanocomposites offered excellent enrichment ability, which was attributed to the synergistic effect of polyethylenimine and silver nanoparticles. The nanocomposites showed superior specificity for glycopeptides even when non-glycopeptides were 100 times more concentrated than glycopeptides. The nanocomposites displayed advantages including rapid adsorption (1 min), low detection limit (25 fmol), repeatability (6 times), and high recovery (77.8%). Using these nanocomposites, 91 different glycoproteins and 136 N-linked glycopeptides were identified from among 20 μg tryptic human serum proteins and this demonstrated the superior performance of the nanocomposites for glycopeptides enrichment.
Starting Page	1607
Ending Page	1615
Page Count	9
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	9
Issue Number	4
Journal	Nanoscale
DOI	10.1039/c6nr09260h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Polyethylenimine Adsorption Graphene Mass spectrometry
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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